{"id":343,"date":"2025-08-29T22:55:36","date_gmt":"2025-08-29T14:55:36","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=343"},"modified":"2025-08-29T22:55:36","modified_gmt":"2025-08-29T14:55:36","slug":"22rv1%e4%ba%ba%e5%89%8d%e5%88%97%e8%85%ba%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/22rv1%e4%ba%ba%e5%89%8d%e5%88%97%e8%85%ba%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"22RV1\u4eba\u524d\u5217\u817a\u764c\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<h2 class=\"header-vfC6AV auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li><strong>\u7ec6\u80de\u540d\u79f0<\/strong>\uff1a22RV1 \u4eba\u524d\u5217\u817a\u764c\u7ec6\u80de\uff08STR \u9274\u5b9a\u6b63\u786e\uff09<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6838\u5fc3\u7279\u8272\uff1a\u524d\u5217\u817a\u6765\u6e90\u764c\u7ec6\u80de\uff08STR \u9274\u5b9a\u6b63\u786e\uff09+ \u8d34\u58c1\u751f\u957f\uff08\u8d34\u58c1\u53ca\u751f\u957f\u6162\uff0c\u4f20\u4ee3\u540e 48h \u5185\u5fcc\u79fb\u52a8\uff09+ \u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001 + 90% DMEM+10% FBS+PS \u57f9\u517b\u57fa +~40 \u5c0f\u65f6\u500d\u589e + \u8868\u8fbe PSA\uff08\u524d\u5217\u817a\u7279\u5f02\u6297\u539f\uff09\u53ca\u96c4\u6fc0\u7d20\u53d7\u4f53 + \u88f8\u9f20\u6210\u7624\u9633\u6027 + \u53ef\u4ea7\u9ad8\u6ef4\u5ea6\u4eba\u9006\u8f6c\u5f55\u75c5\u6bd2 XMRV + \u591a\u673a\u6784\u4fdd\u85cf\uff08ATCC; CRL-2505 \u7b49\uff09\uff0c\u53ef\u4e0e THP-1\uff08\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5 \/ \u60ac\u6d6e \/ 2 &#8211; \u5def\u57fa\u4e59\u9187\u4f9d\u8d56\uff09\u3001TJ905\uff08\u8111\u80f6\u8d28\u7624 \/ \u8d34\u58c1 \/ GFAP \u9633\u6027\uff09\u7ec4\u6210\u5bf9\u6bd4\u4f53\u7cfb\uff0c\u7528\u4e8e\u524d\u5217\u817a\u764c\u673a\u5236\u3001\u5b9e\u4f53\u7624\uff08\u524d\u5217\u817a vs \u8111\uff09vs \u8840\u6db2\u80bf\u7624\u5dee\u5f02\u3001\u96c4\u6fc0\u7d20\u53d7\u4f53\u76f8\u5173\u529f\u80fd\u53ca\u6162\u8d34\u58c1\u7ec6\u80de\u57f9\u517b\u7814\u7a76\u3002<\/li>\n<li><strong>\u7ec6\u80de\u522b\u79f0<\/strong>\uff1a22Rv-1\uff1b\u4eba\u524d\u5217\u817a\u764c\u7ec6\u80de<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6807\u6ce8 \u201c\u524d\u5217\u817a\u764c + \u8d34\u58c1\u6162\uff08\u4f20\u4ee3 48h \u4e0d\u79fb\u52a8\uff09+PSA \u9633\u6027 + DMEM\u201d\uff0c\u533a\u522b\u4e8e\u60ac\u6d6e\u8840\u6db2\u80bf\u7624\uff08THP-1\uff09\u3001\u5feb\u8d34\u58c1\u8111\u80bf\u7624\uff08TJ905\uff09\uff0c\u89c4\u907f\u751f\u957f\u901f\u5ea6\u53ca\u80bf\u7624\u90e8\u4f4d\u6df7\u6dc6\u3002<\/li>\n<li><strong>\u79cd\u5c5e\u6765\u6e90<\/strong>\uff1a\u4eba\uff08STR \u9274\u5b9a\u6b63\u786e\uff0c\u79cd\u5c5e\u7eaf\u51c0\uff0c\u65e0\u4ea4\u53c9\u6c61\u67d3\uff0c\u4fdd\u969c\u524d\u5217\u817a\u764c\u7ec6\u80de\u6162\u8d34\u58c1\u7279\u6027\u3001\u4e0a\u76ae\u5f62\u6001\u3001\u57f9\u517b\u57fa\u9002\u5e94\u53ca PSA \/ \u96c4\u6fc0\u7d20\u53d7\u4f53\u8868\u8fbe\u7a33\u5b9a\u6027\uff0c\u9002\u914d\u524d\u5217\u817a\u764c\u57fa\u7840\u7814\u7a76\u3001\u96c4\u6fc0\u7d20\u9776\u5411\u5b9e\u9a8c\u573a\u666f\uff09<\/li>\n<li><strong>\u7ec4\u7ec7\u6765\u6e90<\/strong>\uff1a\u524d\u5217\u817a\uff08\u524d\u5217\u817a\u764c\u7ec6\u80de\uff09\uff0c\u4fdd\u7559\u524d\u5217\u817a\u764c\u7279\u6709\u7684 PSA \u5206\u6ccc\u53ca\u96c4\u6fc0\u7d20\u53d7\u4f53\u8868\u8fbe\u5c5e\u6027\uff0c\u6838\u5fc3\u4f18\u52bf\u4e3a \u201cPSA \u9633\u6027 + \u96c4\u6fc0\u7d20\u53d7\u4f53\u5173\u8054 + \u6162\u8d34\u58c1\u7279\u6027\u201d\uff0c\u9002\u914d\u524d\u5217\u817a\u764c\u5185\u5206\u6ccc\u6cbb\u7597\u673a\u5236\u3001\u6162\u589e\u6b96\u80bf\u7624\u836f\u7269\u7b5b\u9009\u5b9e\u9a8c\u3002<\/li>\n<li><strong>\u751f\u957f\u7279\u6027<\/strong>\uff1a\u8d34\u58c1\u751f\u957f\uff08\u7eaf\u8d34\u58c1\uff0c\u5173\u952e\u6ce8\u610f\uff1a\u8d34\u58c1\u53ca\u751f\u957f\u6162\uff0c\u4f20\u4ee3 \/ \u6d88\u5316\u540e 48h \u5185\u5fcc\u79fb\u52a8\uff0c\u907f\u514d\u5f71\u54cd\u8d34\u58c1\uff09<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>0.25% \u80f0\u9176 37\u2103\u5b75\u80b2 6-8 \u5206\u949f\u6d88\u5316\uff08\u6162\u8d34\u58c1\u7ec6\u80de\u8d34\u58c1\u8f83\u7262\u56fa\uff0c\u6d88\u5316\u65f6\u95f4\u957f\u4e8e TJ905\uff09\uff0c\u6c47\u5408\u5ea6 70%-80% \u65f6\u6309 1:2 \u4f20\u4ee3\uff08\u751f\u957f\u6162\uff0c\u4f20\u4ee3\u95f4\u9694\u957f\u4e8e\u591a\u6570\u8d34\u58c1\u7ec6\u80de\uff09\uff1b\u4f20\u4ee3\u540e\u9759\u7f6e 37\u2103\u57f9\u517b\u7bb1 48h \u518d\u955c\u68c0\uff0c\u671f\u95f4\u4e0d\u79fb\u52a8\u57f9\u517b\u74f6\uff0c24-48h \u5185\u9010\u6b65\u5b8c\u6210\u8d34\u58c1\uff0c\u9002\u5408\u5f00\u5c55\u524d\u5217\u817a\u764c\u6162\u54cd\u5e94\u5b9e\u9a8c\uff08\u5982\u96c4\u6fc0\u7d20\u8c03\u63a7\u7814\u7a76\uff09\u3002<\/li>\n<li><strong>\u7ec6\u80de\u5f62\u6001<\/strong>\uff1a\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u5448\u591a\u8fb9\u5f62\uff0c\u80de\u8d28\u5747\u5300\uff0c\u6392\u5217\u7d27\u5bc6\uff0c\u5177\u524d\u5217\u817a\u764c\u5178\u578b\u4e0a\u76ae\u5f62\u6001\uff0c\u4e0e THP-1 \u7684\u5355\u6838\u7ec6\u80de\u6837\uff08\u60ac\u6d6e\uff09\u3001TJ905 \u7684\u591a\u89d2\u7ec6\u80de\u6837\uff08\u80f6\u8d28\u7624\uff09\u5f62\u6001\u5dee\u5f02\u663e\u8457\uff0c\u53ef\u901a\u8fc7 \u201c\u524d\u5217\u817a\u6765\u6e90 + PSA \u9633\u6027\u201d \u5feb\u901f\u9274\u522b\uff0c\u9002\u914d\u524d\u5217\u817a\u764c\u7ec6\u80de\u5f62\u6001\u5b66\u9a8c\u8bc1\u53ca\u8d34\u58c1\u72b6\u6001\u8bc4\u4f30\u5b9e\u9a8c\uff09<\/li>\n<li><strong>\u80cc\u666f\u7b80\u4ecb<\/strong>\uff1a\u4eba\u524d\u5217\u817a\u764c\u7ec6\u80de\u7cfb\uff08STR \u9274\u5b9a\u6b63\u786e\uff09\uff0c\u7eaf\u8d34\u58c1\u751f\u957f\u5448\u4e0a\u76ae\u7ec6\u80de\u6837\uff0c90% DMEM+10% FBS \u57f9\u517b\u57fa\u53ef\u7a33\u5b9a\u57f9\u517b\uff1b\u5177\u6162\u8d34\u58c1 \/ \u6162\u751f\u957f\u7279\u6027\uff0c\u8868\u8fbe PSA \u53ca\u96c4\u6fc0\u7d20\u53d7\u4f53\uff0c\u4e8c\u7f9f\u57fa\u777e\u4e38\u8102\u916e\u8f7b\u5fae\u4fc3\u751f\u957f\u3001EGF \u4fc3\u751f\u957f\u3001TG\u03b2-1 \u4e0d\u6291\u751f\u957f\uff0c\u53ef\u4ea7 XMRV \u9006\u8f6c\u5f55\u75c5\u6bd2\uff0c\u88f8\u9f20\u63a5\u79cd\u53ef\u6210\u7624\uff1b\u65e0\u767d\u8840\u75c5\u7684\u541e\u566c\u529f\u80fd\uff08THP-1\uff09\u6216\u80f6\u8d28\u7624\u7684 GFAP \u8868\u8fbe\uff08TJ905\uff09\uff0c\u662f\u7814\u7a76\u524d\u5217\u817a\u764c\u3001\u96c4\u6fc0\u7d20\u4fe1\u53f7\u901a\u8def\u53ca\u6162\u589e\u6b96\u80bf\u7624\u7684\u7406\u60f3\u6a21\u578b\uff0c\u53ef\u4e0e\u96c4\u6fc0\u7d20\u975e\u4f9d\u8d56\u6027\u524d\u5217\u817a\u764c\u7ec6\u80de\u5bf9\u6bd4\u63a2\u7d22\u6cbb\u7597\u8010\u836f\u673a\u5236\u3002<\/li>\n<li><strong>STR \u4f4d\u70b9\u4fe1\u606f<\/strong>\uff1aAmelogenin: X,Y\uff1bCSF1PO: 10,11\uff1bD13S317: 9,12\uff1bD16S539: 12\uff1bD5S818: 11,12,13\uff1bD7S820: 9,10,11\uff1bTH01: 6,9.3\uff1bTPOX: 8\uff1bvWA: 15,21\uff08\u53ef\u4e0e\u540c\u7c7b\u524d\u5217\u817a\u764c\u7ec6\u80de STR \u56fe\u8c31\u5bf9\u6bd4\uff0c\u786e\u8ba4\u524d\u5217\u817a\u764c\u8eab\u4efd\uff0c\u6392\u9664\u5176\u4ed6\u90e8\u4f4d\u80bf\u7624\u7ec6\u80de\u6c61\u67d3\uff09<\/li>\n<li><strong>\u7ec6\u80de\u89c4\u683c<\/strong>\uff1a1\u00d710\u2076cells\/T25 \u8d34\u58c1\u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u590d\u82cf\u540e 48h \u8d34\u58c1\u7387\u226580%\uff08\u6162\u8d34\u58c1\uff0c\u9700\u5ef6\u957f\u89c2\u5bdf\u65f6\u95f4\uff09\uff0c\u5b58\u6d3b\u7ec6\u80de\u4e2d PSA \u9633\u6027\u7387\u226585%\uff0c\u9700\u901a\u8fc7 ELISA \u68c0\u6d4b PSA \u5206\u6ccc\u91cf\u786e\u8ba4\u529f\u80fd\u7a33\u5b9a\u3002<\/li>\n<li><strong>\u652f\u539f\u4f53\u68c0\u6d4b<\/strong>\uff1a\u65e0\uff08\u5efa\u8bae\u6bcf 3 \u4ee3 PCR \u590d\u6d4b\uff0c\u6162\u751f\u957f\u7ec6\u80de\u57f9\u517b\u5468\u671f\u957f\uff0c\u907f\u514d\u6c61\u67d3\u5f71\u54cd\u96c4\u6fc0\u7d20\u53d7\u4f53\u5b9e\u9a8c\u53ca PSA \u5206\u6ccc\u6570\u636e\uff09<\/li>\n<li><strong>\u4fdd\u85cf\u673a\u6784<\/strong>\uff1aATCC; CRL-2505\u3001BCRC; 60545\u3001DSMZ; ACC-438 \u7b49\uff08\u591a\u56fd\u9645\u673a\u6784\u4fdd\u85cf\uff0c\u524d\u5217\u817a\u764c\u5c5e\u6027\u3001PSA \/ \u96c4\u6fc0\u7d20\u53d7\u4f53\u8868\u8fbe\u7ecf\u9a8c\u8bc1\uff0c\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u9002\u914d\u5b9e\u9a8c\u5408\u89c4\u6027\u8981\u6c42\uff09<\/li>\n<li><strong>\u57f9\u517b\u57fa<\/strong>\uff1a90% DMEM+10% FBS+PS\uff08\u8840\u6e05\u9009\u4f4e\u5185\u6bd2\u7d20\u226410EU\/mL\uff0c\u5f00\u5c01\u540e 2 \u5468\u5185\u7528\u5b8c\uff0c\u4fdd\u969c\u6162\u8d34\u58c1\u7279\u6027\u53ca PSA \u5206\u6ccc\u7a33\u5b9a\uff0c\u533a\u522b\u4e8e THP-1 \u7684 1640+2 &#8211; \u5def\u57fa\u4e59\u9187\u3001TJ905 \u7684\u57fa\u7840 DMEM\uff0c\u4e0d\u53ef\u6df7\u7528\uff09<\/li>\n<li><strong>\u57f9\u517b\u6761\u4ef6<\/strong>\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff0c\u6bcf 72 \u5c0f\u65f6\u6362\u6db2 1 \u6b21\uff08\u751f\u957f\u6162\uff0c\u8425\u517b\u6d88\u8017\u6162\uff0c\u6362\u6db2\u9891\u7387\u4f4e\u4e8e TJ905\uff09\uff1b\u57f9\u517b\u7bb1\u6e29\u5ea6\u6ce2\u52a8\u9700\u2264\u00b10.5\u2103\uff0c\u9632\u6b62\u6e29\u5ea6\u53d8\u5316\u5f71\u54cd\u96c4\u6fc0\u7d20\u53d7\u4f53\u8868\u8fbe\u3002<\/li>\n<li><strong>\u51bb\u5b58\u6761\u4ef6<\/strong>\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u6db2\u6c2e\u50a8\u5b58\uff082\u00d710\u2076cells\/mL\uff09\uff0c\u51bb\u5b58\u524d\u9700\u786e\u4fdd\u7ec6\u80de\u5b8c\u5168\u8d34\u58c1\uff08\u907f\u514d\u9009\u53d6\u521a\u4f20\u4ee3\u7684\u672a\u7a33\u5b9a\u7ec6\u80de\uff09\uff0c\u6dfb\u52a0 10% FBS \u63d0\u5347\u590d\u82cf\u540e\u8d34\u58c1\u80fd\u529b\uff1b4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103 4 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u6eb6\u89e3\uff0c\u79bb\u5fc3\u540e\u7528\u65b0\u9c9c DMEM \u91cd\u60ac\uff0c\u63a5\u79cd\u540e\u9759\u7f6e 48h \u518d\u89c2\u5bdf\u3002<\/li>\n<li><strong>\u81f4\u7624\u4e0e\u53d7\u4f53\u6297\u539f\u7279\u5f81<\/strong>\uff1a\u2460 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