{"id":344,"date":"2025-08-29T22:58:58","date_gmt":"2025-08-29T14:58:58","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=344"},"modified":"2025-08-29T22:58:58","modified_gmt":"2025-08-29T14:58:58","slug":"5637%e4%ba%ba%e8%86%80%e8%83%b1%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/5637%e4%ba%ba%e8%86%80%e8%83%b1%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"5637\u4eba\u8180\u80f1\u764c\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<h2 class=\"header-vfC6AV auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li><strong>\u7ec6\u80de\u540d\u79f0<\/strong>\uff1a5637 \u4eba\u8180\u80f1\u764c\u7ec6\u80de\uff08STR \u9274\u5b9a\u6b63\u786e\uff09<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6838\u5fc3\u7279\u8272\uff1a\u8180\u80f1\u6765\u6e90\u764c\u7ec6\u80de\uff08STR \u9274\u5b9a\u6b63\u786e\uff09+1974 \u5e74\u5efa\u682a + \u8d34\u58c1\u751f\u957f + \u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001 + 90% RPMI-1640+10% FBS+PS \u57f9\u517b\u57fa +~24-36 \u5c0f\u65f6\u77ed\u500d\u589e + \u88f8\u9f20 100% \u6210\u7624\uff085\/5\uff0c21 \u5929\u5185\uff09+ \u7624\u7403\u5f62\u6210\u80fd\u529b\uff08\u9002\u914d\u9038\u6f20\u4e13\u7528 3D \u80bf\u7624\u7403\u57f9\u517b\u57fa IMC-314\uff09+ \u8868\u8fbe SCF\/IL-1\/IL-6\/G-CSF \u7b49\u751f\u957f\u56e0\u5b50 + \u67d3\u8272\u4f53 49~64 + \u591a\u673a\u6784\u4fdd\u85cf\uff08ATCC; HTB-9 \u7b49\uff09\uff0c\u53ef\u4e0e 22RV1\uff08\u524d\u5217\u817a\u764c \/ \u8d34\u58c1 \/ DMEM \/ \u6162\u500d\u589e\uff09\u3001THP-1\uff08\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5 \/ \u60ac\u6d6e \/ 2 &#8211; 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\u5fae\u73af\u5883\u6a21\u62df\u7814\u7a76\u3002<\/li>\n<li><strong>\u751f\u957f\u7279\u6027<\/strong>\uff1a\u8d34\u58c1\u751f\u957f\uff08\u7eaf\u8d34\u58c1\uff0c\u64cd\u4f5c\u7b80\u4fbf\uff09<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>0.25% \u80f0\u9176 37\u2103\u5b75\u80b2 3-5 \u5206\u949f\u6d88\u5316\uff08\u77ed\u500d\u589e\u7ec6\u80de\u8d34\u58c1\u4e2d\u7b49\u7d27\u5bc6\uff0c\u6d88\u5316\u65f6\u95f4\u77ed\u4e8e 22RV1\uff09\uff0c\u6c47\u5408\u5ea6 80%-90% \u65f6\u6309 1:3-1:4 \u4f20\u4ee3\uff08\u77ed\u500d\u589e\u9002\u914d\u9ad8\u9891\u4f20\u4ee3\uff09\uff0c24 \u5c0f\u65f6\u5185\u8d34\u58c1\u7a33\u5b9a\uff1b3D \u57f9\u517b\u65f6\uff08IMC-314 \u57f9\u517b\u57fa\uff09\u53ef\u5f62\u6210\u7624\u7403\uff0c\u9002\u5408\u540c\u6b65\u5f00\u5c55 2D\/3D \u5b9e\u9a8c\u5bf9\u6bd4\u3002<\/li>\n<li><strong>\u7ec6\u80de\u5f62\u6001<\/strong>\uff1a\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u5448\u591a\u8fb9\u5f62\uff0c\u80de\u8d28\u5747\u5300\uff0c\u6392\u5217\u7d27\u5bc6\uff0c\u5177\u8180\u80f1\u764c\u5178\u578b\u4e0a\u76ae\u5f62\u6001\uff0c\u4e0e 22RV1 \u7684\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u524d\u5217\u817a\u764c\uff0c\u6162\u8d34\u58c1\uff09\u3001THP-1 \u7684\u5355\u6838\u7ec6\u80de\u6837\uff08\u60ac\u6d6e\uff09\u5f62\u6001\u5dee\u5f02\u663e\u8457\uff0c\u53ef\u901a\u8fc7 \u201c\u8180\u80f1\u6765\u6e90 + \u77ed\u500d\u589e\u201d \u5feb\u901f\u9274\u522b\uff0c\u9002\u914d\u8180\u80f1\u764c\u5f62\u6001\u5b66\u9a8c\u8bc1\u53ca 3D \u7624\u7403\u5f62\u6001\u89c2\u5bdf\u5b9e\u9a8c\uff09<\/li>\n<li><strong>\u80cc\u666f\u7b80\u4ecb<\/strong>\uff1a\u4eba\u8180\u80f1\u764c\u7ec6\u80de\u7cfb\uff08STR \u9274\u5b9a\u6b63\u786e\uff09\uff0c1974 \u5e74\u5206\u79bb\u81ea\u8180\u80f1\u764c\u7ec4\u7ec7\uff0c\u7eaf\u8d34\u58c1\u751f\u957f\u5448\u4e0a\u76ae\u7ec6\u80de\u6837\uff0c90% RPMI-1640+10% FBS \u57f9\u517b\u57fa\u53ef\u7a33\u5b9a\u57f9\u517b\uff1b\u8868\u8fbe SCF\u3001IL-1\u3001IL-6 \u7b49\u751f\u957f\u56e0\u5b50\uff0c\u88f8\u9f20\u76ae\u4e0b\u63a5\u79cd 1\u00d710\u2077\u7ec6\u80de 21 \u5929\u5185 100% \u6210\u7624\uff0c\u5728 IMC-314 \u57f9\u517b\u57fa\u4e2d\u53ef\u5f62\u6210 3D \u7624\u7403\uff1b\u65e0 22RV1 \u7684\u6162\u8d34\u58c1\u7279\u6027\u6216 THP-1 \u7684\u60ac\u6d6e \/ 2 &#8211; \u5def\u57fa\u4e59\u9187\u4f9d\u8d56\uff0c\u662f\u7814\u7a76\u8180\u80f1\u764c\u30013D \u80bf\u7624\u6a21\u578b\u53ca\u6ccc\u5c3f\u751f\u6b96\u7cfb\u7edf\u80bf\u7624\u5dee\u5f02\u7684\u7406\u60f3\u6a21\u578b\uff0c\u53ef\u4e0e\u524d\u5217\u817a\u764c\u7ec6\u80de\u5bf9\u6bd4\u63a2\u7d22\u6ccc\u5c3f\u80bf\u7624\u836f\u7269\u654f\u611f\u6027\u5dee\u5f02\u3002<\/li>\n<li><strong>STR \u4f4d\u70b9\u4fe1\u606f<\/strong>\uff1aAmelogenin\uff1aX\uff0cY\uff1bCSF1PO\uff1a11\uff1bD13S317\uff1a11\uff1bD16S539\uff1a9\uff1bD18S51\uff1a16\uff0c18\uff1bD19S433\uff1a13\uff0c15\uff1bD21S11\uff1a36\uff1bD2S1338\uff1a25\uff1bD3S1358\uff1a15\uff0c17\uff1bD5S818\uff1a11\uff0c12\uff1bD7S820\uff1a10\uff0c11\uff1bD8S1179\uff1a10\uff0c16\uff1bFGA\uff1a22\uff1bTH01\uff1a7\uff0c9\uff1bTPOX\uff1a8\uff1bvWA\uff1a18\uff08\u53ef\u4e0e\u540c\u7c7b\u8180\u80f1\u764c\u7ec6\u80de STR \u56fe\u8c31\u5bf9\u6bd4\uff0c\u786e\u8ba4\u8180\u80f1\u764c\u8eab\u4efd\uff0c\u6392\u9664\u524d\u5217\u817a\u6216\u8840\u6db2\u80bf\u7624\u6c61\u67d3\uff09<\/li>\n<li><strong>\u7ec6\u80de\u89c4\u683c<\/strong>\uff1a1\u00d710\u2076cells\/T25 \u8d34\u58c1\u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u590d\u82cf\u540e\u5b58\u6d3b\u7387\u226585%\uff0c24 \u5c0f\u65f6\u8d34\u58c1\u7387\u226590%\uff1b3D \u57f9\u517b\uff08IMC-314 \u57f9\u517b\u57fa\uff0972 \u5c0f\u65f6\u7624\u7403\u5f62\u6210\u7387\u226575%\uff0c\u9700\u901a\u8fc7 ELISA \u68c0\u6d4b IL-6 \u7b49\u751f\u957f\u56e0\u5b50\u5206\u6ccc\u91cf\uff08\u786e\u4fdd\u529f\u80fd\u7a33\u5b9a\uff09\u3002<\/li>\n<li><strong>\u652f\u539f\u4f53\u68c0\u6d4b<\/strong>\uff1a\u65e0\uff08\u5efa\u8bae\u6bcf 2 \u4ee3 PCR \u590d\u6d4b\uff0c\u77ed\u500d\u589e\u7ec6\u80de\u57f9\u517b\u5468\u671f\u77ed\uff0c\u907f\u514d\u6c61\u67d3\u5f71\u54cd 3D \u7624\u7403\u5f62\u6210\u53ca\u6210\u7624\u5b9e\u9a8c\u7ed3\u679c\uff09<\/li>\n<li><strong>\u4fdd\u85cf\u673a\u6784<\/strong>\uff1aATCC; HTB-9\u3001DSMZ; ACC-35\uff08\u56fd\u9645\u6743\u5a01\u4fdd\u85cf\uff0c\u8180\u80f1\u764c\u5c5e\u6027\u3001\u81f4\u7624\u6027\u53ca\u67d3\u8272\u4f53\u7279\u5f81\u7ecf\u9a8c\u8bc1\uff0c\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u9002\u914d\u5b9e\u9a8c\u7ec6\u80de\u6765\u6e90\u5408\u89c4\u6027\u8981\u6c42\uff09<\/li>\n<li><strong>\u57f9\u517b\u57fa<\/strong>\uff1a90% RPMI-1640+10% FBS+PS\uff08\u8840\u6e05\u9009\u4f4e\u5185\u6bd2\u7d20\u226410EU\/mL\uff0c\u5f00\u5c01\u540e 2 \u5468\u5185\u7528\u5b8c\uff0c\u4fdd\u969c\u77ed\u500d\u589e\u3001\u751f\u957f\u56e0\u5b50\u8868\u8fbe\u53ca\u7624\u7403\u5f62\u6210\u80fd\u529b\uff0c\u533a\u522b\u4e8e 22RV1 \u7684 DMEM\u3001THP-1 \u7684 1640+2 &#8211; \u5def\u57fa\u4e59\u9187\uff09<\/li>\n<li><strong>\u57f9\u517b\u6761\u4ef6<\/strong>\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff1b2D \u57f9\u517b\u6bcf 24-36 \u5c0f\u65f6\u6362\u6db2 1 \u6b21\uff08\u77ed\u500d\u589e\u8425\u517b\u6d88\u8017\u5feb\uff09\uff0c3D \u57f9\u517b\uff08IMC-314 \u57f9\u517b\u57fa\uff09\u6bcf 72 \u5c0f\u65f6\u6362\u6db2 1 \u6b21\uff1b\u9700\u6bcf 12 \u5c0f\u65f6\u955c\u68c0 2D 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