{"id":349,"date":"2025-08-30T07:41:12","date_gmt":"2025-08-29T23:41:12","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=349"},"modified":"2025-08-30T07:41:12","modified_gmt":"2025-08-29T23:41:12","slug":"caki-2%e4%ba%ba%e8%82%be%e9%80%8f%e6%98%8e%e7%bb%86%e8%83%9e%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/caki-2%e4%ba%ba%e8%82%be%e9%80%8f%e6%98%8e%e7%bb%86%e8%83%9e%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"Caki-2\u4eba\u80be\u900f\u660e\u7ec6\u80de\u764c\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<h2 class=\"header-vfC6AV auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li><strong>\u7ec6\u80de\u540d\u79f0<\/strong>\uff1aCaki-2 \u4eba\u80be\u900f\u660e\u7ec6\u80de\u764c\u7ec6\u80de\uff08STR \u9274\u5b9a\u6b63\u786e\uff09<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6838\u5fc3\u7279\u8272\uff1a\u80be\u900f\u660e\u7ec6\u80de\u764c\uff0869 \u5c81\u7537\u6027\u80be\u817a\u764c\u6765\u6e90\uff0cSTR \u9274\u5b9a\u6b63\u786e\uff09+ \u8d34\u58c1\u751f\u957f + \u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001 + McCoy\u2019s 5A+10% FBS+PS \u57f9\u517b\u57fa +~30-40 \u5c0f\u65f6\u500d\u589e + \u88f8\u9f20\u6210\u7624\uff08\u900f\u660e\u7ec6\u80de\u764c\u7c7b\u578b\uff09+ \u67d3\u8272\u4f53\u4e9a\u4e94\u500d\u4f53 \/ \u4e9a\u516d\u500d\u4f53 + \u8840\u578b A\u3001Rh-+ \u591a\u673a\u6784\u4fdd\u85cf\uff08ATCC; HTB-47 \u7b49\uff09\uff0c\u53ef\u4e0e U2OS\uff08\u9aa8\u8089\u7624 \/ \u8d34\u58c1 \/ McCoy\u2019s 5A\/IGF \u53d7\u4f53\u9633\u6027\uff09\u30015637\uff08\u8180\u80f1\u764c \/ \u8d34\u58c1 \/ 1640 \/ \u77ed\u500d\u589e\uff09\u7ec4\u6210\u5bf9\u6bd4\u4f53\u7cfb\uff0c\u7528\u4e8e\u80be\u900f\u660e\u7ec6\u80de\u764c\u673a\u5236\u3001\u6ccc\u5c3f\u751f\u6b96\u7cfb\u7edf\u80bf\u7624\uff08\u80be vs \u8180\u80f1\uff09vs \u9aa8\u80bf\u7624\u5dee\u5f02\u3001McCoy\u2019s 5A \u57f9\u517b\u57fa\u9002\u914d\u6027\u53ca\u80be\u764c\u7279\u5f02\u6027\u7814\u7a76\u3002<\/li>\n<li><strong>\u7ec6\u80de\u522b\u79f0<\/strong>\uff1aCaki-2\uff1b\u4eba\u80be\u900f\u660e\u7ec6\u80de\u764c\u7ec6\u80de<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6807\u6ce8 \u201c\u80be\u900f\u660e\u7ec6\u80de\u764c + \u8d34\u58c1 + McCoy\u2019s 5A+30-40h \u500d\u589e + \u88f8\u9f20\u6210\u900f\u660e\u7ec6\u80de\u764c\u201d\uff0c\u533a\u522b\u4e8e\u540c\u57f9\u517b\u57fa\u7684\u9aa8\u8089\u7624\uff08U2OS\uff09\u30011640 \u57f9\u517b\u7684\u8180\u80f1\u764c\uff085637\uff09\uff0c\u89c4\u907f\u80bf\u7624\u7c7b\u578b\u53ca\u57f9\u517b\u57fa\u6df7\u6dc6\u3002<\/li>\n<li><strong>\u79cd\u5c5e\u6765\u6e90<\/strong>\uff1a\u4eba\uff08STR \u9274\u5b9a\u6b63\u786e\uff0c\u79cd\u5c5e\u7eaf\u51c0\uff0c\u65e0\u4ea4\u53c9\u6c61\u67d3\uff0c\u4fdd\u969c\u80be\u900f\u660e\u7ec6\u80de\u764c\u8d34\u58c1\u80fd\u529b\u3001\u4e0a\u76ae\u5f62\u6001\u3001McCoy\u2019s 5A \u9002\u5e94\u53ca\u81f4\u7624\u6027 \/ \u67d3\u8272\u4f53\u7a33\u5b9a\u6027\uff0c\u9002\u914d\u80be\u764c\u57fa\u7840\u7814\u7a76\u3001\u9776\u5411\u5b9e\u9a8c\u573a\u666f\uff09<\/li>\n<li><strong>\u7ec4\u7ec7\u6765\u6e90<\/strong>\uff1a\u80be\uff08\u80be\u900f\u660e\u7ec6\u80de\u764c\uff0c\u6e90\u81ea\u521d\u671f\u80be\u817a\u764c\uff09\uff0c\u4fdd\u7559\u80be\u764c\u7279\u6709\u7684\u900f\u660e\u7ec6\u80de\u5206\u5316\u7279\u5f81\u53ca\u6076\u6027\u5c5e\u6027\uff0c\u6838\u5fc3\u4f18\u52bf\u4e3a \u201c\u660e\u786e\u80be\u764c\u4e9a\u578b + McCoy\u2019s 5A \u9002\u914d + \u77ed\u500d\u589e\u81f4\u7624\u201d\uff0c\u9002\u914d\u80be\u764c\u53d1\u75c5\u673a\u5236\u3001\u9776\u5411\u836f\u7269\u7b5b\u9009\u53ca\u6ccc\u5c3f\u80bf\u7624\u5bf9\u6bd4\u5b9e\u9a8c\u3002<\/li>\n<li><strong>\u751f\u957f\u7279\u6027<\/strong>\uff1a\u8d34\u58c1\u751f\u957f\uff08\u7eaf\u8d34\u58c1\uff0c\u64cd\u4f5c\u7b80\u4fbf\uff09<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>0.25% \u80f0\u9176 37\u2103\u5b75\u80b2 3-5 \u5206\u949f\u6d88\u5316\uff08\u77ed\u500d\u589e\u7ec6\u80de\u8d34\u58c1\u4e2d\u7b49\u7d27\u5bc6\uff0c\u6d88\u5316\u65f6\u95f4\u77ed\u4e8e\u6210\u7ea4\u7ef4\u7ec6\u80de\uff09\uff0c\u6c47\u5408\u5ea6 80%-90% \u65f6\u6309 1:3 \u4f20\u4ee3\uff0830-40 \u5c0f\u65f6\u500d\u589e\u9002\u914d\u9ad8\u9891\u4f20\u4ee3\uff09\uff0c24 \u5c0f\u65f6\u5185\u8d34\u58c1\u7a33\u5b9a\uff0c\u9002\u5408\u5feb\u901f\u5f00\u5c55\u80be\u764c\u589e\u6b96\u53ca\u81f4\u7624\u76f8\u5173\u5b9e\u9a8c\u3002<\/li>\n<li><strong>\u7ec6\u80de\u5f62\u6001<\/strong>\uff1a\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u5448\u591a\u8fb9\u5f62\uff0c\u80de\u8d28\u900f\u4eae\uff08\u5177\u900f\u660e\u7ec6\u80de\u7279\u5f81\uff09\uff0c\u6392\u5217\u7d27\u5bc6\uff0c\u4e0e U2OS \u7684\u4e0a\u76ae\u6837\uff08\u9aa8\u8089\u7624\uff0c\u80de\u8d28\u9971\u6ee1\uff09\u30015637 \u7684\u4e0a\u76ae\u6837\uff08\u8180\u80f1\u764c\uff0c\u5747\u5300\u80de\u8d28\uff09\u5f62\u6001\u76f8\u6bd4\uff0c\u53ef\u901a\u8fc7 \u201c\u80be\u900f\u660e\u7ec6\u80de\u764c\u6765\u6e90 + McCoy\u2019s 5A\u201d \u5feb\u901f\u9274\u522b\uff0c\u9002\u914d\u80be\u764c\u5f62\u6001\u5b66\u9a8c\u8bc1\u53ca\u4e9a\u578b\u7279\u5f81\u5173\u8054\u5b9e\u9a8c\uff09<\/li>\n<li><strong>\u80cc\u666f\u7b80\u4ecb<\/strong>\uff1a\u4eba\u80be\u900f\u660e\u7ec6\u80de\u764c\u7ec6\u80de\u7cfb\uff08STR \u9274\u5b9a\u6b63\u786e\uff09\uff0c\u6e90\u81ea 69 \u5c81\u767d\u4eba\u7537\u6027\u521d\u671f\u80be\u817a\u764c\uff0c\u7eaf\u8d34\u58c1\u751f\u957f\u5448\u4e0a\u76ae\u7ec6\u80de\u6837\uff0cMcCoy\u2019s 5A+10% FBS \u57f9\u517b\u57fa\u53ef\u7a33\u5b9a\u57f9\u517b\uff1b\u88f8\u9f20\u63a5\u79cd\u5f62\u6210\u900f\u660e\u7ec6\u80de\u764c\uff0c\u67d3\u8272\u4f53\u5448\u4e9a\u4e94\u500d\u4f53 \/ \u4e9a\u516d\u500d\u4f53\uff0c\u65e0 U2OS \u7684 IGF \u53d7\u4f53\u8868\u8fbe\u6216 5637 \u7684\u7624\u7403\u5f62\u6210\u80fd\u529b\uff0c\u662f\u7814\u7a76\u80be\u900f\u660e\u7ec6\u80de\u764c\u3001\u6ccc\u5c3f\u80bf\u7624\u5dee\u5f02\u53ca McCoy\u2019s 5A \u57f9\u517b\u57fa\u9002\u914d\u6027\u7684\u7406\u60f3\u6a21\u578b\uff0c\u53ef\u4e0e\u5176\u4ed6\u80be\u764c\u4e9a\u578b\u5bf9\u6bd4\u63a2\u7d22\u4e9a\u578b\u7279\u5f02\u6027\u673a\u5236\u3002<\/li>\n<li><strong>STR \u4f4d\u70b9\u4fe1\u606f<\/strong>\uff1aAmelogenin\uff1aX\uff0cY\uff1bCSF1PO\uff1a10\uff0c12\uff1bD13S317\uff1a10\uff1bD16S539\uff1a9\uff0c13\uff1bD18S51\uff1a17\uff1bD19S433\uff1a13\uff0c14\uff1bD21S11\uff1a27\uff0c31\uff1bD2S1338\uff1a17\uff0c20\uff1bD3S1358\uff1a14\uff1bD5S818\uff1a11\uff1bD7S820\uff1a12\uff1bD8S1179\uff1a10\uff1bFGA\uff1a22\uff1bTH01\uff1a6\uff1bTPOX\uff1a9\uff0c11\uff1bvWA\uff1a16\uff0c17\uff08\u53ef\u4e0e\u540c\u7c7b\u80be\u764c\u7ec6\u80de STR \u56fe\u8c31\u5bf9\u6bd4\uff0c\u786e\u8ba4\u900f\u660e\u7ec6\u80de\u764c\u8eab\u4efd\uff0c\u6392\u9664\u9aa8\u8089\u7624 \/ \u8180\u80f1\u764c\u6c61\u67d3\uff09<\/li>\n<li><strong>\u7ec6\u80de\u89c4\u683c<\/strong>\uff1a1\u00d710\u2076cells\/T25 \u8d34\u58c1\u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u590d\u82cf\u540e\u5b58\u6d3b\u7387\u226585%\uff0c24 \u5c0f\u65f6\u8d34\u58c1\u7387\u226590%\uff0c\u9700\u955c\u68c0\u786e\u8ba4\u900f\u660e\u7ec6\u80de\u6837\u4e0a\u76ae\u5f62\u6001\u5360\u6bd4\uff08\u226585%\uff09\u3002<\/li>\n<li><strong>\u652f\u539f\u4f53\u68c0\u6d4b<\/strong>\uff1a\u65e0\uff08\u5efa\u8bae\u6bcf 2 \u4ee3 PCR \u590d\u6d4b\uff0c\u77ed\u500d\u589e\u7ec6\u80de\u57f9\u517b\u5468\u671f\u77ed\uff0c\u907f\u514d\u6c61\u67d3\u5f71\u54cd\u80be\u764c\u81f4\u7624\u53ca\u67d3\u8272\u4f53\u5b9e\u9a8c\u7ed3\u679c\uff09<\/li>\n<li><strong>\u4fdd\u85cf\u673a\u6784<\/strong>\uff1aATCC; HTB-47\u3001DSMZ; ACC-54\u3001ECACC; 93120819 \u7b49\uff08\u591a\u56fd\u9645\u673a\u6784\u4fdd\u85cf\uff0c\u80be\u900f\u660e\u7ec6\u80de\u764c\u5c5e\u6027\u3001\u81f4\u7624\u6027\u53ca\u67d3\u8272\u4f53\u7279\u5f81\u7ecf\u9a8c\u8bc1\uff0c\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u9002\u914d\u5b9e\u9a8c\u5408\u89c4\u6027\u8981\u6c42\uff09<\/li>\n<li><strong>\u57f9\u517b\u57fa<\/strong>\uff1aMcCoy\u2019s 5A+10% FBS+PS\uff08\u8840\u6e05\u9009\u4f4e\u5185\u6bd2\u7d20\u226410EU\/mL\uff0c\u5f00\u5c01\u540e 2 \u5468\u5185\u7528\u5b8c\uff0c\u4fdd\u969c\u900f\u660e\u7ec6\u80de\u5f62\u6001\u53ca\u81f4\u7624\u6027\u7a33\u5b9a\uff1b\u4e0e U2OS \u5171\u7528\u57fa\u7840\u57f9\u517b\u57fa\uff0c\u4f46\u4e0d\u53ef\u6df7\u7528\uff08\u907f\u514d\u80bf\u7624\u7c7b\u578b\u4ea4\u53c9\u6c61\u67d3\uff09\uff0c\u533a\u522b\u4e8e 5637 \u7684 RPMI-1640\uff09<\/li>\n<li><strong>\u57f9\u517b\u6761\u4ef6<\/strong>\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff0c\u6bcf 36 \u5c0f\u65f6\u6362\u6db2 1 \u6b21\uff08\u77ed\u500d\u589e\u8425\u517b\u6d88\u8017\u5feb\uff0c\u6362\u6db2\u9891\u7387\u4ecb\u4e8e U2OS \u4e0e 5637 \u4e4b\u95f4\uff09\uff1b\u9700\u6bcf 12 \u5c0f\u65f6\u955c\u68c0\uff0c\u907f\u514d\u8fc7\u5ea6\u6c47\u5408\u5f71\u54cd\u7ec6\u80de\u900f\u660e\u5316\u5f62\u6001\u53ca\u6d3b\u6027\u3002<\/li>\n<li><strong>\u51bb\u5b58\u6761\u4ef6<\/strong>\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u6db2\u6c2e\u50a8\u5b58\uff082\u00d710\u2076cells\/mL\uff09\uff0c4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103 2 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\uff1b\u51bb\u5b58\u524d\u9700\u9009\u53d6\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u900f\u660e\u7ec6\u80de\u7279\u5f81\u6700\u660e\u663e\uff09\uff0c\u6dfb\u52a0 10% FBS \u63d0\u5347\u590d\u82cf\u5b58\u6d3b\u7387\uff0c\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u6eb6\u89e3\uff0c\u79bb\u5fc3\u540e\u7528\u65b0\u9c9c McCoy\u2019s 5A \u57f9\u517b\u57fa\u91cd\u60ac\u3002<\/li>\n<li><strong>\u81f4\u7624\u4e0e\u67d3\u8272\u4f53\u7279\u5f81<\/strong>\uff1a\u2460 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