{"id":361,"date":"2025-08-30T10:17:10","date_gmt":"2025-08-30T02:17:10","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=361"},"modified":"2025-08-30T10:17:11","modified_gmt":"2025-08-30T02:17:11","slug":"kmh-2%e4%ba%ba%e7%94%b2%e7%8a%b6%e8%85%ba%e7%99%8c%e7%bb%86%e8%83%9e%e6%9c%aa%e5%88%86%e5%8c%96","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/kmh-2%e4%ba%ba%e7%94%b2%e7%8a%b6%e8%85%ba%e7%99%8c%e7%bb%86%e8%83%9e%e6%9c%aa%e5%88%86%e5%8c%96\/","title":{"rendered":"KMH-2\u4eba\u7532\u72b6\u817a\u764c\u7ec6\u80de(\u672a\u5206\u5316)"},"content":{"rendered":"<h2 class=\"header-vfC6AV auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li><strong>\u7ec6\u80de\u540d\u79f0<\/strong>\uff1aKMH-2 \u4eba\u7532\u72b6\u817a\u764c\u7ec6\u80de (\u672a\u5206\u5316)<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6838\u5fc3\u7279\u8272\uff1a71 \u5c81\u7537\u6027\u7532\u72b6\u817a\u672a\u5206\u5316\uff08\u95f4\u53d8\u6027\uff09\u764c\uff08ATC\uff0c\u6076\u6027\u7a0b\u5ea6\u9ad8\uff09+ \u8d34\u58c1\u751f\u957f + \u68ad\u5f62\u7ec6\u80de\u5f62\u6001 + 90% DMEM+10% FBS \u57f9\u517b\u57fa +~58 \u5c0f\u65f6\u500d\u589e\uff08PubMed=11686581 \u6587\u732e\u652f\u6301\uff09+ \u53cc\u673a\u6784\u4fdd\u85cf\uff08\u4e2d\u56fd\u79d1\u5b66\u9662\u5206\u5b50\u7ec6\u80de\u79d1\u5b66\u5353\u8d8a\u521b\u65b0\u4e2d\u5fc3\u3001JCRB; JCRB1066\uff09\uff0c\u53ef\u4e0e TPC-1\uff08\u7532\u72b6\u817a\u4e73\u5934\u72b6\u764c \/ \u8d34\u58c1 \/ \u4e0a\u76ae\u7ec6\u80de\u6837 \/ \u4f4e\u6076\u6027\uff09\u3001A-427\uff08\u80ba\u764c \/ \u8d34\u58c1 \/ \u4e0a\u76ae\u7ec6\u80de\u6837 \/ KRAS \u7a81\u53d8\uff09\u7ec4\u6210\u5bf9\u6bd4\u4f53\u7cfb\uff0c\u7528\u4e8e\u7532\u72b6\u817a\u672a\u5206\u5316\u764c\u673a\u5236\u7814\u7a76\u3001\u7532\u72b6\u817a\u764c\u4e9a\u578b\uff08\u672a\u5206\u5316 vs \u5206\u5316\u578b\uff09\u5dee\u5f02\u3001\u68ad\u5f62\u7ec6\u80de\u80bf\u7624 vs \u4e0a\u76ae\u6837\u80bf\u7624\u5bf9\u6bd4\u53ca ATC \u9776\u5411\u836f\u7269\u7b5b\u9009\u5b9e\u9a8c\u3002<\/li>\n<li><strong>\u7ec6\u80de\u522b\u79f0<\/strong>\uff1aKMH-2\uff1b\u4eba\u7532\u72b6\u817a\u764c\u7ec6\u80de<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6807\u6ce8 \u201c\u7532\u72b6\u817a\u672a\u5206\u5316\u764c\uff08ATC\uff09+ \u8d34\u58c1 + \u68ad\u5f62\u7ec6\u80de + DMEM+58h \u500d\u589e\u201d\uff0c\u533a\u522b\u4e8e\u5206\u5316\u578b\u7532\u72b6\u817a\u764c\u7ec6\u80de\uff08TPC-1\uff09\u3001\u4e0a\u76ae\u6837\u80ba\u764c\u7ec6\u80de\uff08A-427\uff09\uff0c\u89c4\u907f\u80bf\u7624\u5206\u5316\u7a0b\u5ea6\u3001\u5f62\u6001\u53ca\u90e8\u4f4d\u6df7\u6dc6\u3002<\/li>\n<li><strong>\u79cd\u5c5e\u6765\u6e90<\/strong>\uff1a\u4eba\uff08\u79cd\u5c5e\u7eaf\u51c0\u65e0\u4ea4\u53c9\u6c61\u67d3\uff0c\u4fdd\u969c\u7532\u72b6\u817a\u672a\u5206\u5316\u764c\u7ec6\u80de\u8d34\u58c1\u80fd\u529b\u3001\u68ad\u5f62\u5f62\u6001\u3001DMEM \u57f9\u517b\u57fa\u9002\u5e94\u53ca\u500d\u589e\u7a33\u5b9a\u6027\uff0c\u9002\u914d\u9ad8\u6076\u6027\u7532\u72b6\u817a\u764c\u57fa\u7840\u7814\u7a76\u3001\u6297\u80bf\u7624\u5b9e\u9a8c\u573a\u666f\uff09<\/li>\n<li><strong>\u7ec4\u7ec7\u6765\u6e90<\/strong>\uff1a\u7532\u72b6\u817a\uff08\u672a\u5206\u5316\uff08\u95f4\u53d8\u6027\uff09\u764c\uff0cATC\uff09\uff0c\u6e90\u81ea 71 \u5c81\u7537\u6027\u60a3\u8005\uff0c\u4fdd\u7559 ATC \u7279\u6709\u7684\u9ad8\u6076\u6027\u589e\u6b96\u7279\u5f81\u3001\u68ad\u5f62\u7ec6\u80de\u5f62\u6001\u53ca\u4f4e\u5206\u5316\u5c5e\u6027\uff0c\u6838\u5fc3\u4f18\u52bf\u4e3a \u201c\u660e\u786e\u672a\u5206\u5316\u764c\u4e9a\u578b + \u68ad\u5f62\u7ec6\u80de\u5178\u578b\u5f62\u6001 + \u6587\u732e\u652f\u6301\u500d\u589e\u201d\uff0c\u9002\u914d\u7532\u72b6\u817a\u764c\u6076\u6027\u8fdb\u5c55\u673a\u5236\u3001\u9ad8\u4fb5\u88ad\u6027\u80bf\u7624\u5b9e\u9a8c\u7814\u7a76\u3002<\/li>\n<li><strong>\u751f\u957f\u7279\u6027<\/strong>\uff1a\u8d34\u58c1\u751f\u957f\uff08\u7eaf\u8d34\u58c1\uff0c\u68ad\u5f62\u7ec6\u80de\u8d34\u58c1\u8f83\u7d27\u5bc6\uff09<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>0.25% \u80f0\u9176 37\u2103\u5b75\u80b2 6-8 \u5206\u949f\u6d88\u5316\uff08\u68ad\u5f62\u7ec6\u80de\u8d34\u58c1\u5f3a\u5ea6\u9ad8\u4e8e\u4e0a\u76ae\u6837\u7ec6\u80de\uff0c\u9700\u5ef6\u957f\u6d88\u5316\u65f6\u95f4\uff09\uff0c\u6c47\u5408\u5ea6 70%-80% \u65f6\u6309 1:2 \u4f20\u4ee3\uff08\u500d\u589e\u8f83\u6162\uff0c\u4f20\u4ee3\u95f4\u9694\u957f\u4e8e\u5206\u5316\u578b\u7532\u72b6\u817a\u764c\u7ec6\u80de\uff09\uff0c24-48 \u5c0f\u65f6\u5185\u7a33\u5b9a\u8d34\u58c1\uff0c\u9002\u5408\u5f00\u5c55 ATC \u6162\u589e\u6b96\u76f8\u5173\u5b9e\u9a8c\uff08\u5982\u4fb5\u88ad\u8f6c\u79fb\u3001\u836f\u7269\u8010\u836f\u7814\u7a76\uff09\u3002<\/li>\n<li><strong>\u7ec6\u80de\u5f62\u6001<\/strong>\uff1a\u68ad\u5f62\u7ec6\u80de\uff08\u5448\u957f\u68ad\u5f62\uff0c\u80de\u4f53\u8212\u5c55\uff0c\u6392\u5217\u5448\u653e\u5c04\u72b6\u6216\u6f29\u6da1\u72b6\uff0c\u5177\u672a\u5206\u5316\u764c\u5178\u578b\u68ad\u5f62\u7279\u5f81\uff0c\u4e0e TPC-1 \u7684 \u201c\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u591a\u8fb9\u5f62\uff0c\u7d27\u5bc6\u6392\u5217\uff09\u201d\u3001A-427 \u7684 \u201c\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u591a\u8fb9\u5f62\uff0c\u80de\u8d28\u5747\u5300\uff09\u201d \u5f62\u6001\u5dee\u5f02\u663e\u8457\uff0c\u53ef\u901a\u8fc7 \u201c\u7532\u72b6\u817a\u672a\u5206\u5316\u764c\u6765\u6e90 + \u68ad\u5f62\u201d \u5feb\u901f\u9274\u522b\uff0c\u9002\u914d ATC \u5f62\u6001\u5b66\u9a8c\u8bc1\u53ca\u7eaf\u5ea6\u68c0\u6d4b\u5b9e\u9a8c\uff09<\/li>\n<li><strong>\u80cc\u666f\u7b80\u4ecb<\/strong>\uff1a\u4eba\u7532\u72b6\u817a\u672a\u5206\u5316\uff08\u95f4\u53d8\u6027\uff09\u764c\u7ec6\u80de\u7cfb\uff0c\u7eaf\u8d34\u58c1\u751f\u957f\u5448\u68ad\u5f62\u7ec6\u80de\uff0c90% DMEM+10% FBS \u57f9\u517b\u57fa\u53ef\u7a33\u5b9a\u57f9\u517b\uff1b\u6e90\u81ea 71 \u5c81\u7537\u6027 ATC \u60a3\u8005\uff0c\u500d\u589e\u65f6\u95f4\uff5e58 \u5c0f\u65f6\uff08\u6587\u732e\u4f50\u8bc1\uff09\uff0c\u65e0 TPC-1 \u7684\u5206\u5316\u578b\u7532\u72b6\u817a\u764c\u7279\u5f81\uff08\u5982\u6444\u7898\u80fd\u529b\uff09\u6216 A-427 \u7684\u80ba\u764c KRAS \u7a81\u53d8\u5c5e\u6027\uff0c\u662f\u7814\u7a76\u7532\u72b6\u817a\u672a\u5206\u5316\u764c\u9ad8\u6076\u6027\u673a\u5236\u3001\u7532\u72b6\u817a\u764c\u4e9a\u578b\u5dee\u5f02\u7684\u7406\u60f3\u6a21\u578b\uff0c\u53ef\u4e0e\u5206\u5316\u578b\u7532\u72b6\u817a\u764c\u7ec6\u80de\u5bf9\u6bd4\u63a2\u7d22\u6076\u6027\u8f6c\u5316\u5173\u952e\u901a\u8def\u3002<\/li>\n<li><strong>STR \u4f4d\u70b9\u4fe1\u606f<\/strong>\uff1aAmelogenin\uff1aX\uff0cY\uff1bD5S818: 12,13\uff1bD13S317: 9\uff1bD7S820: 11\uff1bD16S539: 9,12\uff1bvWA: 14,15\uff1bTHO1: 9\uff1bTPOX: 8,11\uff1bCSF1PO: 10,11\uff08\u53ef\u4e0e\u540c\u7c7b\u7532\u72b6\u817a\u764c\u7ec6\u80de STR \u56fe\u8c31\u5bf9\u6bd4\uff0c\u786e\u8ba4 KMH-2 \u7ec6\u80de\u8eab\u4efd\uff0c\u6392\u9664\u5206\u5316\u578b\u7532\u72b6\u817a\u764c\u6216\u5176\u4ed6\u90e8\u4f4d\u80bf\u7624\u7ec6\u80de\u6c61\u67d3\uff09<\/li>\n<li><strong>\u7ec6\u80de\u89c4\u683c<\/strong>\uff1a1\u00d710\u2076cells\/T25 \u8d34\u58c1\u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u590d\u82cf\u540e\u5b58\u6d3b\u7387\u226580%\uff0c48 \u5c0f\u65f6\u8d34\u58c1\u7387\u226590%\uff0c\u9700\u955c\u68c0\u786e\u8ba4\u68ad\u5f62\u7ec6\u80de\u5f62\u6001\u5360\u6bd4\uff08\u226585%\uff09\uff0c\u6392\u9664\u4e0a\u76ae\u6837\u6742\u7ec6\u80de\u6c61\u67d3\u3002<\/li>\n<li><strong>\u652f\u539f\u4f53\u68c0\u6d4b<\/strong>\uff1a\u65e0\uff08\u7ecf PCR \u6cd5\u6216\u57f9\u517b\u6cd5\u68c0\u6d4b\uff0c\u786e\u8ba4\u65e0\u652f\u539f\u4f53\u6c61\u67d3\uff0c\u907f\u514d\u5f71\u54cd ATC \u7ec6\u80de\u589e\u6b96\u53ca\u4fb5\u88ad\u5b9e\u9a8c\u7ed3\u679c\u51c6\u786e\u6027\uff09<\/li>\n<li><strong>\u4fdd\u85cf\u673a\u6784<\/strong>\uff1a\u4e2d\u56fd\u79d1\u5b66\u9662\u5206\u5b50\u7ec6\u80de\u79d1\u5b66\u5353\u8d8a\u521b\u65b0\u4e2d\u5fc3\u3001JCRB; JCRB1066\uff08\u56fd\u5185\u5916\u6743\u5a01\u673a\u6784\u4fdd\u85cf\uff0cKMH-2 \u7532\u72b6\u817a\u672a\u5206\u5316\u764c\u5c5e\u6027\u3001\u5f62\u6001\u53ca\u500d\u589e\u7279\u5f81\u7ecf\u9a8c\u8bc1\uff0c\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u9002\u914d\u5b9e\u9a8c\u7ec6\u80de\u6765\u6e90\u5408\u89c4\u6027\u8981\u6c42\uff09<\/li>\n<li><strong>\u57f9\u517b\u57fa<\/strong>\uff1a90% DMEM+10% FBS\uff08\u8840\u6e05\u9009\u4f4e\u5185\u6bd2\u7d20\u226410EU\/mL\uff0c\u5f00\u5c01\u540e 2 \u5468\u5185\u7528\u5b8c\uff0c\u4fdd\u969c\u68ad\u5f62\u7ec6\u80de\u5f62\u6001\u53ca\u9ad8\u6076\u6027\u7279\u5f81\u7a33\u5b9a\uff1b\u533a\u522b\u4e8e TPC-1 \u7684\u57fa\u7840 DMEM\uff08\u65e0\u7279\u6b8a\u5dee\u5f02\u4f46\u9700\u533a\u5206\u4e9a\u578b\uff09\u3001A-427 \u7684 MEM+NEAA \u590d\u5408\u57f9\u517b\u57fa\uff0c\u4e0d\u53ef\u6df7\u7528\uff09<\/li>\n<li><strong>\u57f9\u517b\u6761\u4ef6<\/strong>\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff1b\u6bcf 72 \u5c0f\u65f6\u6362\u6db2 1 \u6b21\uff08\u500d\u589e\u6162\uff0c\u8425\u517b\u6d88\u8017\u7f13\uff0c\u6362\u6db2\u9891\u7387\u4f4e\u4e8e\u5206\u5316\u578b\u80bf\u7624\u7ec6\u80de\uff09\uff1b\u6cbf\u57f9\u517b\u74f6\u58c1\u7f13\u6162\u52a0\u6db2\uff0c\u907f\u514d\u51b2\u51fb\u8d34\u58c1\u7684\u68ad\u5f62\u7ec6\u80de\uff0c\u9632\u6b62\u7ec6\u80de\u8131\u843d\u3002<\/li>\n<li><strong>\u51bb\u5b58\u6761\u4ef6<\/strong>\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\uff08-196\u2103\uff09\u4e2d\u957f\u671f\u50a8\u5b58\uff1b\u51bb\u5b58\u524d\u9700\u9009\u53d6\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u68ad\u5f62\u7279\u5f81\u6700\u660e\u663e\uff09\uff0c\u6dfb\u52a0 10% FBS \u63d0\u5347\u590d\u82cf\u5b58\u6d3b\u7387\uff1b\u68af\u5ea6\u964d\u6e29\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 4 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u8f6c\u5165\u6db2\u6c2e\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u6eb6\u89e3\uff0c\u79bb\u5fc3\u540e\u7528\u65b0\u9c9c DMEM \u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u590d\u82cf\u540e 48 \u5c0f\u65f6\u955c\u68c0\u8d34\u58c1\u72b6\u6001\u3002<\/li>\n<li><strong>\u500d\u589e\u65f6\u95f4<\/strong>\uff1a~58 \u5c0f\u65f6\uff08PubMed \u6587\u732e\u7f16\u53f7 11686581 \u9a8c\u8bc1\uff0c\u9002\u914d ATC \u6162\u589e\u6b96\u7279\u6027\u7814\u7a76\uff0c\u533a\u522b\u4e8e TPC-1\uff08~24-36 \u5c0f\u65f6\uff09\u7684\u5feb\u500d\u589e\uff0c\u53ef\u7528\u4e8e\u5bf9\u6bd4\u5206\u5316\u7a0b\u5ea6\u4e0e\u589e\u6b96\u901f\u5ea6\u7684\u5173\u8054\uff09<\/li>\n<li><strong>\u7ec6\u80de\u8d27\u671f<\/strong>\uff1a\u73b0\u8d27\uff0c\u4e0b\u5355\u540e 1 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