{"id":371,"date":"2025-08-30T17:52:05","date_gmt":"2025-08-30T09:52:05","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=371"},"modified":"2025-08-30T17:52:05","modified_gmt":"2025-08-30T09:52:05","slug":"nci-h838%e4%ba%ba%e9%9d%9e%e5%b0%8f%e7%bb%86%e8%83%9e%e8%82%ba%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/nci-h838%e4%ba%ba%e9%9d%9e%e5%b0%8f%e7%bb%86%e8%83%9e%e8%82%ba%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"NCI-H838\u4eba\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<h2 class=\"header-vfC6AV auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li><strong>\u7ec6\u80de\u540d\u79f0<\/strong>\uff1aNCI-H838 \u4eba\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u7ec6\u80de<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6838\u5fc3\u7279\u8272\uff1a59 \u5c81\u7537\u6027\u767d\u4eba\u5438\u70df\u8005\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\uff08\u6dcb\u5df4\u7ed3\u8f6c\u79fb\u7076\uff0c1984 \u5e74\u5efa\u7cfb\uff09+ \u8d34\u58c1\u751f\u957f + \u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001 + RPMI-1640+10% FBS+PS \u57f9\u517b\u57fa +~55 \u5c0f\u65f6\u500d\u589e + ATCC CRL-5844 \u4fdd\u85cf\uff0c\u53ef\u4e0e A549\uff08\u975e\u5c0f\u7ec6\u80de\u80ba\u764c \/ \u8d34\u58c1 \/ RPMI-1640 \/ \u500d\u589e\uff5e24 \u5c0f\u65f6 \/ \u65e0\u5438\u70df\u80cc\u666f\uff09\u3001NCI-H1299\uff08\u975e\u5c0f\u7ec6\u80de\u80ba\u764c \/ \u8d34\u58c1 \/ RPMI-1640 \/ \u65e0 EGFR \u7a81\u53d8 \/ \u539f\u53d1\u7076\u6765\u6e90\uff09\u7ec4\u6210\u5bf9\u6bd4\u4f53\u7cfb\uff0c\u7528\u4e8e\u5438\u70df\u8005\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u673a\u5236\u7814\u7a76\u3001\u80ba\u764c\u8f6c\u79fb\u7076 vs \u539f\u53d1\u7076\u5dee\u5f02\u3001\u6162\u500d\u589e\u80ba\u764c\u7ec6\u80de\u57f9\u517b\u53ca\u6297\u80ba\u764c\u836f\u7269\u7b5b\u9009\u5b9e\u9a8c\u3002<\/li>\n<li><strong>\u7ec6\u80de\u522b\u79f0<\/strong>\uff1aH838\uff1bH-838\uff1bNCIH838<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6807\u6ce8 \u201c\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\uff08\u6dcb\u5df4\u7ed3\u8f6c\u79fb\u7076\uff0c59 \u5c81\u7537\u6027\u5438\u70df\u8005\uff09+ \u8d34\u58c1\u4e0a\u76ae\u6837 + RPMI-1640+55h 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\u5c81\uff08\u660e\u786e\u60a3\u8005\u5e74\u9f84\u6027\u522b\u53ca\u5438\u70df\u53f2\uff0c\u9002\u914d\u80ba\u764c\u6027\u522b\u76f8\u5173\u673a\u5236\u3001\u5438\u70df\u81f4\u80ba\u764c\u75c5\u7406\u751f\u7406\u7814\u7a76\uff09<\/li>\n<li><strong>\u7ec4\u7ec7\u6765\u6e90<\/strong>\uff1a\u80ba\uff08\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\uff0c\u6e90\u4e8e 59 \u5c81\u767d\u4eba\u7537\u6027\u5438\u70df\u8005\u7684\u6dcb\u5df4\u7ed3\u8f6c\u79fb\u7076\uff0c1984 \u5e74\u5efa\u7cfb\uff09\uff0c\u4fdd\u7559\u80ba\u764c\u8f6c\u79fb\u7076\u7279\u6709\u7684\u4fb5\u88ad\u5c5e\u6027\u3001\u5438\u70df\u8005\u80ba\u764c\u5206\u5b50\u7279\u5f81\uff08\u5982\u6f5c\u5728\u70df\u8349\u76f8\u5173\u57fa\u56e0\u7a81\u53d8\uff09\uff0c\u6838\u5fc3\u4f18\u52bf\u4e3a \u201c\u8f6c\u79fb\u7076\u6765\u6e90 + \u5438\u70df\u4e34\u5e8a\u80cc\u666f + \u660e\u786e\u500d\u589e\u65f6\u95f4\u201d\uff0c\u9002\u914d\u80ba\u764c\u8f6c\u79fb\u673a\u5236\u3001\u5438\u70df\u76f8\u5173\u80ba\u764c\u5206\u5b50\u901a\u8def\u7814\u7a76\u3002<\/li>\n<li><strong>\u751f\u957f\u7279\u6027<\/strong>\uff1a\u8d34\u58c1\u751f\u957f\uff08\u7eaf\u8d34\u58c1\uff0c\u6162\u500d\u589e\u9700\u63a7\u5236\u4f20\u4ee3\u95f4\u9694\uff09<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>0.25% \u80f0\u9176 37\u2103\u5b75\u80b2 4-6 \u5206\u949f\u6d88\u5316\uff08\u4e0a\u76ae\u7ec6\u80de\u8d34\u58c1\u4e2d\u7b49\u7d27\u5bc6\uff0c\u6162\u500d\u589e\u7ec6\u80de\u6d88\u5316\u654f\u611f\u6027\u7565\u4f4e\uff0c\u9700\u907f\u514d\u8fc7\u5ea6\u6d88\u5316\uff09\uff0c\u6c47\u5408\u5ea6 70%-80% \u65f6\u6309 1:2 \u4f20\u4ee3\uff08\u56e0\u500d\u589e\uff5e55 \u5c0f\u65f6\uff0c\u4f20\u4ee3\u95f4\u9694\u957f\u4e8e A549\uff0c\u7ea6 3-4 \u5929\u4f20\u4ee3 1 \u6b21\uff09\uff1b\u7ec6\u80de\u5bc6\u5ea6\u7ef4\u6301\u5728 1\u00d710\u2074-3\u00d710\u2074cells\/cm\u00b2\uff08\u5bc6\u5ea6\u8fc7\u4f4e\u6613\u51cb\u4ea1\uff0c\u8fc7\u9ad8\u6613\u51fa\u73b0\u63a5\u89e6\u6291\u5236\uff09\uff0c\u6bcf 48 \u5c0f\u65f6\u6362\u6db2 1 \u6b21\uff0c\u9002\u5408\u5f00\u5c55\u80ba\u764c\u8f6c\u79fb\u76f8\u5173\u6162\u53cd\u5e94\u5b9e\u9a8c\uff08\u5982\u4fb5\u88ad\u8fc1\u79fb\u957f\u6548\u76d1\u6d4b\uff09\u3002<\/li>\n<li><strong>\u7ec6\u80de\u5f62\u6001<\/strong>\uff1a\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u5448\u591a\u8fb9\u5f62\uff0c\u80de\u8d28\u5747\u5300\uff0c\u6392\u5217\u8f83\u677e\u6563\uff08\u8f6c\u79fb\u7076\u7ec6\u80de\u7279\u5f81\uff09\uff0c\u4e0e A549 \u7684 \u201c\u89c4\u5219\u4e0a\u76ae\u6837\uff08\u6392\u5217\u7d27\u5bc6\uff0c\u77ed\u500d\u589e\uff09\u201d\u3001NCI-H1299 \u7684 \u201c\u4e0a\u76ae\u6837\uff08\u7565\u68ad\u5f62\uff0c\u539f\u53d1\u7076\uff09\u201d \u5f62\u6001\u5dee\u5f02\u663e\u8457\uff0c\u53ef\u901a\u8fc7 \u201c\u6dcb\u5df4\u7ed3\u8f6c\u79fb\u7076\u6765\u6e90 + \u5438\u70df\u80cc\u666f\u201d \u5feb\u901f\u9274\u522b\uff0c\u9002\u914d\u80ba\u764c\u8f6c\u79fb\u7076\u5f62\u6001\u5b66\u9a8c\u8bc1\u53ca\u7eaf\u5ea6\u68c0\u6d4b\u5b9e\u9a8c\uff09<\/li>\n<li><strong>\u80cc\u666f\u7b80\u4ecb<\/strong>\uff1a\u4eba\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u7ec6\u80de\u7cfb\uff0c1984 \u5e74\u5efa\u7cfb\uff0c\u7eaf\u8d34\u58c1\u751f\u957f\u5448\u4e0a\u76ae\u7ec6\u80de\u6837\uff0cRPMI-1640+10% FBS+PS \u57f9\u517b\u57fa\u53ef\u7a33\u5b9a\u57f9\u517b\uff1b\u6e90\u4e8e 59 \u5c81\u767d\u4eba\u7537\u6027\u5438\u70df\u8005\u7684\u6dcb\u5df4\u7ed3\u8f6c\u79fb\u7076\uff0c\u5177\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u5178\u578b\u6076\u6027\u7279\u5f81\uff0c\u65e0 A549 \u7684\u77ed\u500d\u589e\u5c5e\u6027\u6216 NCI-H1299 \u7684\u539f\u53d1\u7076\u7279\u5f81\uff1b\u56e0\u643a\u5e26\u5438\u70df\u76f8\u5173\u80ba\u764c\u6f5c\u5728\u5206\u5b50\u6807\u8bb0\uff0c\u4e14\u4e3a\u8f6c\u79fb\u7076\u6765\u6e90\uff0c\u662f\u7814\u7a76\u5438\u70df\u8005\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u3001\u80ba\u764c\u6dcb\u5df4\u7ed3\u8f6c\u79fb\u673a\u5236\u53ca\u6162\u500d\u589e\u80ba\u764c\u7ec6\u80de\u836f\u7269\u654f\u611f\u6027\u7684\u7406\u60f3\u6a21\u578b\uff0c\u53ef\u4e0e\u975e\u5438\u70df\u80ba\u764c\u7ec6\u80de\u5bf9\u6bd4\u63a2\u7d22\u5438\u70df\u81f4\u80ba\u764c\u7684\u7279\u5f02\u6027\u901a\u8def\u3002<\/li>\n<li><strong>STR \u4f4d\u70b9\u4fe1\u606f<\/strong>\uff1aAmelogenin: X\uff1bCSF1PO: 10\uff1bD13S317: 8\uff1bD16S539: 9,12\uff1bD5S818: 11\uff1bD7S820: 9\uff1bTHO1: 7\uff1bTPOX: 8,11\uff1bvWA: 17\uff08\u53ef\u4e0e ATCC CRL-5844 \u6807\u51c6 STR \u56fe\u8c31\u6bd4\u5bf9\uff0c\u786e\u8ba4\u8f6c\u79fb\u7076\u7ec6\u80de\u8eab\u4efd\uff0c\u6392\u9664\u975e\u5438\u70df\u80ba\u764c\u7ec6\u80de\u6216\u5176\u4ed6\u90e8\u4f4d\u80bf\u7624\u7ec6\u80de\u6c61\u67d3\uff09<\/li>\n<li><strong>\u7ec6\u80de\u89c4\u683c<\/strong>\uff1a1\u00d710\u2076cells\/T25 \u8d34\u58c1\u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u590d\u82cf\u540e\u5b58\u6d3b\u7387\u226580%\uff0c48 \u5c0f\u65f6\u8d34\u58c1\u7387\u226590%\uff0c\u9700\u955c\u68c0\u786e\u8ba4\u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001\u5360\u6bd4\uff08\u226585%\uff09\uff0c\u6392\u9664\u68ad\u5f62\u6742\u7ec6\u80de\u6c61\u67d3\u3002<\/li>\n<li><strong>\u652f\u539f\u4f53\u68c0\u6d4b<\/strong>\uff1a\u65e0\uff08\u7ecf PCR \u6cd5\u6216\u57f9\u517b\u6cd5\u68c0\u6d4b\uff0c\u786e\u8ba4\u65e0\u652f\u539f\u4f53\u6c61\u67d3\uff0c\u907f\u514d\u5f71\u54cd\u80ba\u764c\u7ec6\u80de\u8f6c\u79fb\u76f8\u5173\u5b9e\u9a8c\uff08\u5982 Transwell \u4fb5\u88ad\uff09\u53ca\u836f\u7269\u654f\u611f\u6027\u7ed3\u679c\u51c6\u786e\u6027\uff09<\/li>\n<li><strong>\u4fdd\u85cf\u673a\u6784<\/strong>\uff1aATCC; CRL-5844\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\u4fdd\u85cf\uff0cNCI-H838 \u7ec6\u80de\u8f6c\u79fb\u7076\u5c5e\u6027\u3001\u5438\u70df\u80cc\u666f\u5173\u8054\u7279\u5f81\u53ca\u500d\u589e\u65f6\u95f4\u7ecf\u9a8c\u8bc1\uff0c\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u9002\u914d\u5b9e\u9a8c\u7ec6\u80de\u6765\u6e90\u5408\u89c4\u6027\u8981\u6c42\uff09<\/li>\n<li><strong>\u57f9\u517b\u57fa<\/strong>\uff1aRPMI-1640 \u57f9\u517b\u57fa + 10% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff0c\u6d53\u5ea6 100U\/mL \u9752\u9709\u7d20 + 100\u03bcg\/mL \u94fe\u9709\u7d20\uff09\uff1b\u8840\u6e05\u9009\u4f4e\u5185\u6bd2\u7d20\u226410EU\/mL\uff08\u5438\u70df\u76f8\u5173\u7ec6\u80de\u5bf9\u8840\u6e05\u8d28\u91cf\u654f\u611f\uff0c\u9ad8\u5185\u6bd2\u7d20\u6613\u5bfc\u81f4\u7ec6\u80de\u5e94\u6fc0\u53cd\u5e94\uff09\uff0c\u5f00\u5c01\u540e 2 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E-cadherin\u3001Vimentin\uff09\u3002<\/li>\n<li><strong>\u51bb\u5b58\u6761\u4ef6<\/strong>\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\uff08-196\u2103\uff09\u4e2d\u957f\u671f\u50a8\u5b58\uff1b\u51bb\u5b58\u524d\u9700\u9009\u53d6\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u6162\u500d\u589e\u7ec6\u80de\u5bf9\u6570\u671f\u6301\u7eed\u65f6\u95f4\u957f\uff0c\u9700\u63d0\u524d\u89c2\u5bdf\u786e\u8ba4\uff09\uff0c\u6dfb\u52a0 10% FBS \u63d0\u5347\u590d\u82cf\u5b58\u6d3b\u7387\uff1b\u7ec6\u80de\u6d53\u5ea6 2\u00d710\u2076cells\/mL\uff0c\u68af\u5ea6\u964d\u6e29\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103 4 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u8f6c\u5165\u6db2\u6c2e\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u6eb6\u89e3\uff0c\u79bb\u5fc3\u540e\u7528\u65b0\u9c9c RPMI-1640 \u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u590d\u82cf\u540e 72 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