{"id":382,"date":"2025-09-01T22:34:02","date_gmt":"2025-09-01T14:34:02","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=382"},"modified":"2025-09-01T22:34:02","modified_gmt":"2025-09-01T14:34:02","slug":"hs578bst%e4%ba%ba%e6%ad%a3%e5%b8%b8%e4%b9%b3%e8%85%ba%e7%bb%86%e8%83%9e","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/hs578bst%e4%ba%ba%e6%ad%a3%e5%b8%b8%e4%b9%b3%e8%85%ba%e7%bb%86%e8%83%9e\/","title":{"rendered":"Hs578Bst\u4eba\u6b63\u5e38\u4e73\u817a\u7ec6\u80de"},"content":{"rendered":"<h2 class=\"header-vfC6AV auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li><strong>\u7ec6\u80de\u540d\u79f0<\/strong>\uff1aHs 578Bst \u4eba\u6b63\u5e38\u4e73\u817a\u7ec6\u80de<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6838\u5fc3\u7279\u8272\uff1a74 \u5c81\u5973\u6027\u6b63\u5e38\u4e73\u817a\u7ec4\u7ec7\uff08\u6d78\u6da6\u6027\u5bfc\u7ba1\u764c\u65c1\uff0c\u4e0e Hs 578T \u540c\u6e90\uff09+ \u8d34\u58c1\u751f\u957f + \u6210\u7ea4\u7ef4\u7ec6\u80de\u6837\u5f62\u6001 + DMEM+10% FBS+1% \u53cc\u6297\u57f9\u517b\u57fa + 10 \u4ee3\u4ee5\u5185\u4f4e\u4ee3\u6570 + \u808c\u4e0a\u76ae\u8d77\u6e90\uff08\u5fae\u4e1d\u3001\u80de\u996e\u6db2\u6ce1\u7279\u5f81\uff09+ \u65e0\u96cc\u6fc0\u7d20\u53d7\u4f53 + \u8868\u8fbe EGF \u53d7\u4f53 + ATCC HTB-125 \u4fdd\u85cf\uff0c\u53ef\u4e0e Hs 578T\uff08\u540c\u6e90\u4e73\u817a\u764c\u7ec6\u80de \/ \u8d34\u58c1 \/ DMEM \/ \u6709\u764c\u7279\u5f81\uff09\u3001MCF-7\uff08\u4e73\u817a\u764c\u7ec6\u80de \/ \u8d34\u58c1 \/ DMEM\/ER \u9633\u6027\uff09\u7ec4\u6210\u5bf9\u6bd4\u4f53\u7cfb\uff0c\u7528\u4e8e\u4e73\u817a\u764c\u673a\u5236\u7814\u7a76\uff08\u6b63\u5e38 vs \u764c\u53d8\u5bf9\u7167\uff09\u3001\u4e73\u817a\u808c\u4e0a\u76ae\u7ec6\u80de\u529f\u80fd\u3001\u65e0 ER \u4e73\u817a\u7ec6\u80de\u5b9e\u9a8c\u53ca\u6297\u4e73\u817a\u764c\u836f\u7269\u7b5b\u9009\u7684\u6b63\u5e38\u7ec6\u80de\u5bf9\u7167\u3002<\/li>\n<li><strong>\u7ec6\u80de\u522b\u79f0<\/strong>\uff1aHs578Bst\uff1bHS578BST\uff1bHs-578Bst\uff1bHs 578.Bst\uff1bHomo sapiens No. 578, breast cells<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6807\u6ce8 \u201c\u6b63\u5e38\u4e73\u817a\uff08\u764c\u65c1\u7ec4\u7ec7\uff0c74 \u5c81\u5973\u6027\uff09+ \u8d34\u58c1\u6210\u7ea4\u7ef4\u6837 + DMEM + \u808c\u4e0a\u76ae\u8d77\u6e90 + \u65e0 ER\u201d\uff0c\u533a\u522b\u4e8e\u540c\u6e90\u764c\u7ec6\u80de\uff08Hs 578T\uff09\u3001ER \u9633\u6027\u4e73\u817a\u764c\u7ec6\u80de\uff08MCF-7\uff09\uff0c\u89c4\u907f \u201c\u6b63\u5e38 vs \u764c\u53d8\u201d\u201c\u808c\u4e0a\u76ae vs \u4e0a\u76ae\u201d \u5c5e\u6027\u6df7\u6dc6\u3002<\/li>\n<li><strong>\u79cd\u5c5e\u6765\u6e90<\/strong>\uff1a\u4eba\uff08\u79cd\u5c5e\u7eaf\u51c0\u65e0\u4ea4\u53c9\u6c61\u67d3\uff0c\u4fdd\u969c\u6b63\u5e38\u4e73\u817a\u7ec6\u80de\u8d34\u58c1\u80fd\u529b\u3001\u6210\u7ea4\u7ef4\u5f62\u6001\u3001DMEM \u57f9\u517b\u57fa\u9002\u5e94\u53ca\u808c\u4e0a\u76ae\u7279\u6027\u7a33\u5b9a\uff0c\u9002\u914d\u4e73\u817a\u764c\u6b63\u5e38\u5bf9\u7167\u3001\u4e73\u817a\u751f\u7406\u529f\u80fd\u7814\u7a76\u573a\u666f\uff09<\/li>\n<li><strong>\u5e74\u9f84\u6027\u522b<\/strong>\uff1a\u5973\uff1b74 \u5c81\uff08\u660e\u786e\u60a3\u8005\u5e74\u9f84\u6027\u522b\u53ca\u764c\u65c1\u80cc\u666f\uff0c\u9002\u914d\u8001\u5e74\u5973\u6027\u4e73\u817a\u751f\u7406\u3001\u4e73\u817a\u764c\u764c\u53d8\u673a\u5236\uff08\u764c\u65c1 vs \u764c\u7ec4\u7ec7\uff09\u7814\u7a76\uff09<\/li>\n<li><strong>\u7ec4\u7ec7\u6765\u6e90<\/strong>\uff1a\u4e73\u817a\uff08\u6b63\u5e38\u4e73\u817a\u7ec4\u7ec7\uff0c\u6e90\u4e8e 74 \u5c81\u5973\u6027\u6d78\u6da6\u6027\u5bfc\u7ba1\u764c\u65c1\u7ec4\u7ec7\uff0c\u4e0e\u764c\u7ec6\u80de\u7cfb Hs 578T \u540c\u6e90\uff09\uff0c\u4fdd\u7559\u6b63\u5e38\u4e73\u817a\u808c\u4e0a\u76ae\u7ec6\u80de\u6838\u5fc3\u7279\u5f81\uff08\u5fae\u4e1d\u7ed3\u6784\u3001\u4e1b\u751f\u80de\u996e\u6db2\u6ce1\uff09\uff0c\u65e0\u764c\u53d8\u5c5e\u6027\uff0c\u6838\u5fc3\u4f18\u52bf\u4e3a \u201c\u764c\u65c1\u6b63\u5e38\u5bf9\u7167 + \u808c\u4e0a\u76ae\u8d77\u6e90 + \u65e0 ER \u8868\u8fbe\u201d\uff0c\u9002\u914d\u4e73\u817a\u764c\u764c\u53d8\u65e9\u671f\u673a\u5236\u3001\u6b63\u5e38\u4e73\u817a\u7ec6\u80de\u529f\u80fd\u8c03\u63a7\u5b9e\u9a8c\u3002<\/li>\n<li><strong>\u751f\u957f\u7279\u6027<\/strong>\uff1a\u8d34\u58c1\u751f\u957f\uff08\u7eaf\u8d34\u58c1\uff0c\u6210\u7ea4\u7ef4\u7ec6\u80de\u6837\u8d34\u58c1\u8f83\u7d27\u5bc6\uff09<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>0.25% \u80f0\u9176 37\u2103\u5b75\u80b2 6-8 \u5206\u949f\u6d88\u5316\uff08\u6210\u7ea4\u7ef4\u7ec6\u80de\u8d34\u58c1\u5f3a\u5ea6\u9ad8\u4e8e\u4e0a\u76ae\u7ec6\u80de\uff0c\u9700\u5ef6\u957f\u6d88\u5316\u65f6\u95f4\uff09\uff0c\u6c47\u5408\u5ea6 70%-80% \u65f6\u6309 1:2 \u4f20\u4ee3\uff08\u6b63\u5e38\u7ec6\u80de\u589e\u6b96\u901f\u7387\u6162\u4e8e\u764c\u7ec6\u80de\uff0c\u4f20\u4ee3\u95f4\u9694 3-4 \u5929\uff09\uff1b\u4f20\u4ee3\u540e 24-48 \u5c0f\u65f6\u7a33\u5b9a\u8d34\u58c1\uff0c\u9700\u7ef4\u6301\u7ec6\u80de\u5bc6\u5ea6\u22651\u00d710\u2074cells\/cm\u00b2\uff08\u5bc6\u5ea6\u8fc7\u4f4e\u6613\u4e22\u5931\u808c\u4e0a\u76ae\u7279\u6027\uff09\uff0c\u9002\u5408\u5f00\u5c55\u6b63\u5e38\u4e73\u817a\u7ec6\u80de\u529f\u80fd\u5b9e\u9a8c\uff08\u5982\u4e0e\u764c\u7ec6\u80de\u5171\u57f9\u517b\u7814\u7a76\u764c\u53d8\u5fae\u73af\u5883\uff09\u3002<\/li>\n<li><strong>\u7ec6\u80de\u5f62\u6001<\/strong>\uff1a\u6210\u7ea4\u7ef4\u7ec6\u80de\u6837\uff08\u5448\u957f\u68ad\u5f62\uff0c\u80de\u4f53\u8212\u5c55\uff0c\u6392\u5217\u5448\u653e\u5c04\u72b6\u6216\u6f29\u6da1\u72b6\uff0c\u5177\u808c\u4e0a\u76ae\u7ec6\u80de\u5178\u578b\u6210\u7ea4\u7ef4\u5f62\u6001\uff0c\u4e0e Hs 578T \u7684 \u201c\u4e0a\u76ae\u6837\uff08\u764c\u53d8\u7279\u5f81\uff0c\u6392\u5217\u677e\u6563\uff09\u201d\u3001MCF-7 \u7684 \u201c\u4e0a\u76ae\u6837\uff08ER \u9633\u6027\uff0c\u7d27\u5bc6\u6392\u5217\uff09\u201d \u5f62\u6001\u5dee\u5f02\u663e\u8457\uff0c\u53ef\u901a\u8fc7 \u201c\u764c\u65c1\u6b63\u5e38\u6765\u6e90 + \u6210\u7ea4\u7ef4\u6837\u201d \u5feb\u901f\u9274\u522b\uff0c\u9002\u914d\u6b63\u5e38\u4e73\u817a\u7ec6\u80de\u5f62\u6001\u5b66\u9a8c\u8bc1\u53ca\u7eaf\u5ea6\u5b9e\u9a8c\uff09<\/li>\n<li><strong>\u80cc\u666f\u7b80\u4ecb<\/strong>\uff1a\u4eba\u6b63\u5e38\u4e73\u817a\u7ec6\u80de\u7cfb\uff0c\u6e90\u4e8e 74 \u5c81\u5973\u6027\u6d78\u6da6\u6027\u5bfc\u7ba1\u764c\u65c1\u7ec4\u7ec7\uff08\u4e0e\u764c\u7ec6\u80de\u7cfb Hs 578T \u540c\u6e90\uff09\uff0c\u8d34\u58c1\u751f\u957f\u5448\u6210\u7ea4\u7ef4\u7ec6\u80de\u6837\uff0cDMEM+10% FBS+1% \u53cc\u6297\u57f9\u517b\u57fa\u53ef\u7a33\u5b9a\u57f9\u517b\uff1b\u6838\u5fc3\u7279\u5f81\uff1a\u2460 \u8d77\u6e90\u5c5e\u6027\uff1a\u808c\u4e0a\u76ae\u8d77\u6e90\uff0c\u8d85\u5fae\u7ed3\u6784\u53ef\u89c1\u7c7b\u4f3c\u4f53\u5185\u808c\u4e0a\u76ae\u7684\u5fae\u4e1d\u3001\u4e1b\u751f\u80de\u996e\u6db2\u6ce1\uff0c\u65e0\u6865\u7c92\uff1b\u2461 \u5206\u5b50\u7279\u5f81\uff1a\u65e0\u96cc\u6fc0\u7d20\u53d7\u4f53\u86cb\u767d\uff0c\u8868\u8fbe\u8868\u76ae\u751f\u957f\u56e0\u5b50\uff08EGF\uff09\u53d7\u4f53\uff1b\u2462 \u65e0 Hs 578T \u7684\u764c\u53d8\u7279\u5f81\u6216 MCF-7 \u7684 ER \u9633\u6027\u5c5e\u6027\uff0c\u662f\u7814\u7a76\u4e73\u817a\u764c\u764c\u53d8\u673a\u5236\u3001\u6b63\u5e38\u4e73\u817a\u808c\u4e0a\u76ae\u529f\u80fd\u7684\u7406\u60f3 \u201c\u6b63\u5e38\u5bf9\u7167\u201d \u6a21\u578b\u3002<\/li>\n<li><strong>\u7ec6\u80de\u4ee3\u6570<\/strong>\uff1a10 \u4ee3\u4ee5\u5185\uff08\u4f4e\u4ee3\u6570\u4fdd\u969c\u6b63\u5e38\u4e73\u817a\u7ec6\u80de\u808c\u4e0a\u76ae\u7279\u6027\u3001\u65e0 ER \u8868\u8fbe\u53ca\u589e\u6b96\u7a33\u5b9a\u6027\uff0c\u4f20\u4ee3\u8d85\u8fc7 15 \u4ee3\u9700\u91cd\u65b0\u9a8c\u8bc1\u808c\u4e0a\u76ae\u6807\u5fd7\u7269\uff09<\/li>\n<li><strong>\u751f\u7269\u5b89\u5168\u7b49\u7ea7<\/strong>\uff1a1 \u7ea7\uff08\u64cd\u4f5c\u65e0\u7279\u6b8a\u751f\u7269\u5b89\u5168\u8981\u6c42\uff0c\u9002\u914d\u5e38\u89c4\u7ec6\u80de\u5b9e\u9a8c\u5ba4\u7814\u7a76\uff09<\/li>\n<li><strong>\u7ec6\u80de\u89c4\u683c<\/strong>\uff1a1\u00d710\u2076cells\/T25 \u8d34\u58c1\u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u590d\u82cf\u540e\u5b58\u6d3b\u7387\u226580%\uff0c48 \u5c0f\u65f6\u8d34\u58c1\u7387\u226590%\uff0c\u9700\u955c\u68c0\u786e\u8ba4\u6210\u7ea4\u7ef4\u7ec6\u80de\u6837\u5f62\u6001\u5360\u6bd4\uff08\u226585%\uff09\uff0c\u6392\u9664\u4e0a\u76ae\u6837\u6742\u7ec6\u80de\u6c61\u67d3\u3002<\/li>\n<li><strong>\u652f\u539f\u4f53\u68c0\u6d4b<\/strong>\uff1a\u65e0\uff08\u7ecf PCR \u6cd5\u6216\u57f9\u517b\u6cd5\u68c0\u6d4b\uff0c\u786e\u8ba4\u65e0\u652f\u539f\u4f53\u6c61\u67d3\uff0c\u907f\u514d\u5f71\u54cd\u6b63\u5e38\u4e73\u817a\u7ec6\u80de\u589e\u6b96\u53ca\u808c\u4e0a\u76ae\u529f\u80fd\u5b9e\u9a8c\u7ed3\u679c\uff09<\/li>\n<li><strong>\u4fdd\u85cf\u673a\u6784<\/strong>\uff1aATCC; HTB-125\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\u4fdd\u85cf\uff0c\u7ec6\u80de\u6b63\u5e38\u4e73\u817a\u5c5e\u6027\u3001\u808c\u4e0a\u76ae\u8d77\u6e90\u53ca\u65e0 ER \u7279\u5f81\u7ecf\u9a8c\u8bc1\uff0c\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u9002\u914d\u4e73\u817a\u764c\u7814\u7a76\u7684\u6b63\u5e38\u5bf9\u7167\u7ec6\u80de\u5408\u89c4\u6027\u8981\u6c42\uff09<\/li>\n<li><strong>\u57f9\u517b\u57fa<\/strong>\uff1aDMEM \u57f9\u517b\u57fa + 10% FBS+1% \u53cc\u6297\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff0c\u6d53\u5ea6 100U\/mL \u9752\u9709\u7d20 + 100\u03bcg\/mL \u94fe\u9709\u7d20\uff09\uff1b<strong>\u4e0d\u53ef\u66ff\u6362\u4e3a\u7279\u6b8a\u6dfb\u52a0\u5242\u57f9\u517b\u57fa<\/strong>\uff08\u5e38\u89c4 DMEM \u5373\u53ef\u7ef4\u6301\u6b63\u5e38\u7ec6\u80de\u529f\u80fd\uff0c\u65e0\u9700\u989d\u5916\u6dfb\u52a0\u6fc0\u7d20\u6216\u751f\u957f\u56e0\u5b50\uff09\uff1b\u8840\u6e05\u9009\u4f4e\u5185\u6bd2\u7d20\u226410EU\/mL\uff0c\u5f00\u5c01\u540e 2 \u5468\u5185\u7528\u5b8c\uff0c\u4fdd\u969c\u7ec6\u80de\u5f62\u6001\u53ca\u808c\u4e0a\u76ae\u7279\u6027\u7a33\u5b9a\u3002<\/li>\n<li><strong>\u57f9\u517b\u6761\u4ef6<\/strong>\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff1b\u6bcf 48 \u5c0f\u65f6\u6362\u6db2 1 \u6b21\uff08\u6cbf\u57f9\u517b\u74f6\u58c1\u7f13\u6162\u52a0\u6db2\uff0c\u907f\u514d\u51b2\u51fb\u8d34\u58c1\u7684\u6210\u7ea4\u7ef4\u7ec6\u80de\uff09\uff1b\u57f9\u517b\u7bb1\u6e29\u5ea6\u6ce2\u52a8\u9700\u2264\u00b10.5\u2103\uff0c\u9632\u6b62\u5f71\u54cd EGF \u53d7\u4f53\u8868\u8fbe\u53ca\u808c\u4e0a\u76ae\u7279\u5f81\u3002<\/li>\n<li><strong>\u51bb\u5b58\u6761\u4ef6<\/strong>\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\uff08-196\u2103\uff09\u4e2d\u957f\u671f\u50a8\u5b58\uff1b\u51bb\u5b58\u524d\u9700\u9009\u53d6\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u808c\u4e0a\u76ae\u7279\u6027\u6700\u7a33\u5b9a\uff09\uff0c\u6dfb\u52a0 10% FBS \u63d0\u5347\u590d\u82cf\u5b58\u6d3b\u7387\uff1b\u68af\u5ea6\u964d\u6e29\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103 4 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u8f6c\u5165\u6db2\u6c2e\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u6eb6\u89e3\uff0c\u79bb\u5fc3\u540e\u7528\u65b0\u9c9c DMEM \u57f9\u517b\u57fa\u91cd\u60ac\u3002<\/li>\n<li><strong>\u53d7\u4f53\u8868\u8fbe\u60c5\u51b5<\/strong>\uff1aEpidermal growth factor (EGF) receptor\uff08\u8868\u8fbe EGF \u53d7\u4f53\uff0c\u53c2\u4e0e\u6b63\u5e38\u4e73\u817a\u7ec6\u80de\u589e\u6b96\u3001\u5206\u5316\u8c03\u63a7\uff0c\u9002\u914d EGF \u4fe1\u53f7\u901a\u8def\u7814\u7a76\uff09<\/li>\n<li><strong>\u4ea7\u54c1\u8d27\u671f<\/strong>\uff1a1 \u5468\u5de6\u53f3<\/li>\n<li><strong>\u8fd0\u8f93\u65b9\u5f0f<\/strong>\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u57f9\u517b\u74f6\u5305\u88c5\uff0c\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u8fd0\u8f93\u540e\u9759\u7f6e 48 \u5c0f\u65f6\u518d\u955c\u68c0\uff0c\u907f\u514d\u5e72\u6270\u6b63\u5e38\u7ec6\u80de\u8d34\u58c1\uff09\uff1b\u51bb\u5b58\u53d1\u8d27\uff081mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff0c\u9700\u989d\u5916\u652f\u4ed8\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff09\uff0c\u9ed8\u8ba4\u987a\u4e30\u5feb\u9012\uff08\u4f18\u5148\u51b7\u94fe\u8fd0\u8f93\uff0c\u786e\u4fdd\u6b63\u5e38\u4e73\u817a\u7ec6\u80de\u6d3b\u6027\uff09\u3002<\/li>\n<li><strong>\u4f9b\u5e94\u9650\u5236<\/strong>\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\u4f7f\u7528\uff08\u5305\u62ec\u4e73\u817a\u764c\u764c\u53d8\u673a\u5236\uff08\u6b63\u5e38 vs \u764c\u53d8\u5bf9\u7167\uff09\u3001\u4e73\u817a\u808c\u4e0a\u76ae\u7ec6\u80de\u529f\u80fd\u3001EGF 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