{"id":387,"date":"2025-09-01T22:59:09","date_gmt":"2025-09-01T14:59:09","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=387"},"modified":"2025-09-01T22:59:09","modified_gmt":"2025-09-01T14:59:09","slug":"f56%e4%ba%ba%e8%85%ba%e7%99%8c%e7%bb%86%e8%83%9e%e7%b3%bb%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/f56%e4%ba%ba%e8%85%ba%e7%99%8c%e7%bb%86%e8%83%9e%e7%b3%bb%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"F56\u4eba\u817a\u764c\u7ec6\u80de\u7cfb\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<h2 class=\"header-vfC6AV auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li><strong>\u7ec6\u80de\u540d\u79f0<\/strong>\uff1aF56 \u4eba\u817a\u764c\u7ec6\u80de\u7cfb\uff08STR \u9274\u5b9a\u6b63\u786e\uff09<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6838\u5fc3\u7279\u8272\uff1a\u4eba\u901a\u7528\u578b\u817a\u764c\u7ec6\u80de\uff08\u65e0\u7279\u5b9a\u5668\u5b98\u6807\u6ce8\uff09+ \u8d34\u58c1\u751f\u957f + \u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001 + 90% DMEM+10% FBS \u57f9\u517b\u57fa + 10 \u4ee3\u4ee5\u5185\u4f4e\u4ee3\u6570 +<strong>A \u7c7b\u4f7f\u7528\u6743\u9650<\/strong>+ \u4e2d\u56fd\u5178\u578b\u57f9\u517b\u7269\u4fdd\u85cf\u4e2d\u5fc3\u4fdd\u85cf\uff0c\u53ef\u4e0e A549\uff08\u80ba\u817a\u764c \/ \u8d34\u58c1 \/ F-12K\/10% FBS \/ \u5668\u5b98\u7279\u5f02\u6027\uff09\u3001MCF-7\uff08\u4e73\u817a\u817a\u764c \/ \u8d34\u58c1 \/ DMEM\/10% FBS\/ER \u9633\u6027\uff09\u7ec4\u6210\u5bf9\u6bd4\u4f53\u7cfb\uff0c\u7528\u4e8e\u901a\u7528\u578b\u817a\u764c\u53d1\u751f\u673a\u5236\u7814\u7a76\u3001\u8de8\u5668\u5b98\u817a\u764c\u5171\u6027\u7279\u5f81\u5206\u6790\u3001\u6297\u764c\u836f\u7269\u5e7f\u8c31\u7b5b\u9009\uff08\u9002\u914d\u591a\u7c7b\u578b\u817a\u764c\u836f\u6548\u9a8c\u8bc1\uff09\u53ca\u57fa\u7840\u817a\u764c\u6a21\u578b\u5b9e\u9a8c\u3002<\/li>\n<li><strong>\u7ec6\u80de\u522b\u79f0<\/strong>\uff1aF56\uff1b\u4eba\u817a\u764c\u7ec6\u80de\u7cfb<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6807\u6ce8 \u201c\u4eba\u901a\u7528\u817a\u764c\u7ec6\u80de + \u8d34\u58c1\u4e0a\u76ae\u6837 + DMEM+10% FBS+A \u7c7b\u6743\u9650\u201d\uff0c\u533a\u522b\u4e8e\u5668\u5b98\u7279\u5f02\u6027\u817a\u764c\u7ec6\u80de\uff08A549\/MCF-7\uff09\uff0c\u89c4\u907f \u201c\u901a\u7528\u578b vs \u5668\u5b98\u7279\u5f02\u578b\u201d \u817a\u764c\u6a21\u578b\u6df7\u6dc6\uff0c\u9002\u914d\u65e0\u660e\u786e\u5668\u5b98\u6307\u5411\u7684\u817a\u764c\u57fa\u7840\u7814\u7a76\u3002<\/li>\n<li><strong>\u79cd\u5c5e\u6765\u6e90<\/strong>\uff1a\u4eba\uff08STR \u9274\u5b9a\u6b63\u786e\uff0c\u79cd\u5c5e\u7eaf\u51c0\u65e0\u4ea4\u53c9\u6c61\u67d3\uff0c\u4fdd\u969c\u817a\u764c\u7ec6\u80de\u8d34\u58c1\u80fd\u529b\u3001\u4e0a\u76ae\u5f62\u6001\u3001DMEM \u57f9\u517b\u57fa\u9002\u5e94\u7a33\u5b9a\u6027\uff0c\u9002\u914d\u901a\u7528\u817a\u764c\u673a\u5236\u3001\u6297\u764c\u836f\u7269\u7b5b\u9009\u5b9e\u9a8c\u573a\u666f\uff09<\/li>\n<li><strong>\u7ec4\u7ec7\u6765\u6e90<\/strong>\uff1a\u4eba\u817a\u764c\uff08\u901a\u7528\u578b\u817a\u764c\u7ec6\u80de\uff0c\u65e0\u7279\u5b9a\u5668\u5b98\u6807\u6ce8\uff0c\u4fdd\u7559\u817a\u764c\u6838\u5fc3\u6076\u6027\u7279\u5f81\u5982\u4e0a\u76ae\u5206\u5316\u5c5e\u6027\u3001\u589e\u6b96\u6d3b\u6027\uff0c\u9002\u914d\u8de8\u5668\u5b98\u817a\u764c\u5171\u6027\u673a\u5236\u7814\u7a76\uff09<\/li>\n<li><strong>\u751f\u957f\u7279\u6027<\/strong>\uff1a\u8d34\u58c1\u751f\u957f\uff08\u7eaf\u8d34\u58c1\uff0c\u4e0a\u76ae\u7ec6\u80de\u6837\u8d34\u58c1\u4e2d\u7b49\u7d27\u5bc6\uff0c\u64cd\u4f5c\u9700\u63a7\u5236\u6d88\u5316\u5f3a\u5ea6\uff09<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>0.25% \u80f0\u9176 37\u2103\u5b75\u80b2 3-5 \u5206\u949f\u6d88\u5316\uff08\u907f\u514d\u8fc7\u5ea6\u6d88\u5316\u5bfc\u81f4\u7ec6\u80de\u7834\u788e\uff09\uff0c\u6c47\u5408\u5ea6 80%-90% \u65f6\u6309 1:2-1:3 \u4f20\u4ee3\uff08\u817a\u764c\u7ec6\u80de\u589e\u6b96\u901f\u7387\u4e2d\u7b49\uff0c\u4f20\u4ee3\u95f4\u9694 2-3 \u5929\uff09\uff1b\u7ec6\u80de\u5bc6\u5ea6\u7ef4\u6301\u5728 1\u00d710\u2074-3\u00d710\u2074cells\/cm\u00b2\uff08\u5bc6\u5ea6\u8fc7\u4f4e\u6613\u51cb\u4ea1\uff0c\u8fc7\u9ad8\u6613\u51fa\u73b0\u63a5\u89e6\u6291\u5236\uff09\uff0c\u6bcf 48 \u5c0f\u65f6\u6362\u6db2 1 \u6b21\uff0c\u9002\u5408\u5f00\u5c55\u817a\u764c\u836f\u7269\u654f\u611f\u6027\u68c0\u6d4b\uff08\u5982 MTT \u6cd5\u836f\u6548\u5b9e\u9a8c\uff09\u3002<\/li>\n<li><strong>\u7ec6\u80de\u5f62\u6001<\/strong>\uff1a\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u5448\u89c4\u5219\u591a\u8fb9\u5f62\uff0c\u80de\u8d28\u5747\u5300\uff0c\u6392\u5217\u7d27\u5bc6\uff08\u817a\u764c\u5178\u578b\u4e0a\u76ae\u5f62\u6001\uff09\uff0c\u4e0e A549 \u7684 \u201c\u4e0a\u76ae\u6837\uff08\u80ba\u817a\u764c\u7279\u5f81\uff0c\u7565\u677e\u6563\uff09\u201d\u3001MCF-7 \u7684 \u201c\u4e0a\u76ae\u6837\uff08\u4e73\u817a\u817a\u764c\u7279\u5f81\uff0c\u6392\u5217\u81f4\u5bc6\uff09\u201d \u5f62\u6001\u76f8\u6bd4\uff0c\u5177\u901a\u7528\u817a\u764c\u4e0a\u76ae\u5f62\u6001\u5171\u6027\uff0c\u53ef\u901a\u8fc7 \u201c\u901a\u7528\u578b\u6765\u6e90 + DMEM \u57f9\u517b\u57fa\u201d \u5feb\u901f\u9274\u522b\uff0c\u9002\u914d\u817a\u764c\u5f62\u6001\u5b66\u9a8c\u8bc1\u53ca\u7eaf\u5ea6\u68c0\u6d4b\u5b9e\u9a8c\uff09<\/li>\n<li><strong>\u80cc\u666f\u7b80\u4ecb<\/strong>\uff1a\u4eba\u901a\u7528\u578b\u817a\u764c\u7ec6\u80de\u7cfb\uff08STR \u9274\u5b9a\u6b63\u786e\uff09\uff0c\u8d34\u58c1\u751f\u957f\u5448\u4e0a\u76ae\u7ec6\u80de\u6837\uff0c90% DMEM+10% FBS \u57f9\u517b\u57fa\u53ef\u7a33\u5b9a\u57f9\u517b\uff1b\u6838\u5fc3\u5e94\u7528\u4ef7\u503c\uff1a\u2460 \u9002\u914d\u817a\u764c\u53d1\u751f\u673a\u5236\u7684\u901a\u7528\u578b\u7814\u7a76\uff08\u65e0\u9700\u5c40\u9650\u4e8e\u7279\u5b9a\u5668\u5b98\uff09\uff1b\u2461 \u53ef\u4f5c\u4e3a\u6297\u764c\u836f\u7269\u7684\u5e7f\u8c31\u7b5b\u9009\u6a21\u578b\uff08\u9a8c\u8bc1\u836f\u7269\u5bf9\u591a\u7c7b\u578b\u817a\u764c\u7684\u6f5c\u5728\u6d3b\u6027\uff09\uff1b\u65e0 A549\/MCF-7 \u7684\u5668\u5b98\u7279\u5f02\u6027\u5206\u5b50\u7279\u5f81\uff08\u5982\u80ba\u817a\u764c\u76f8\u5173\u7a81\u53d8\u3001\u4e73\u817a\u817a\u764c ER \u8868\u8fbe\uff09\uff0c\u662f\u7814\u7a76\u817a\u764c\u5171\u6027\u7279\u5f81\u3001\u5f00\u53d1\u5e7f\u8c31\u6297\u764c\u836f\u7269\u7684\u7406\u60f3\u57fa\u7840\u6a21\u578b\u3002<\/li>\n<li><strong>\u7ec6\u80de\u4ee3\u6570<\/strong>\uff1a10 \u4ee3\u4ee5\u5185\uff08\u4f4e\u4ee3\u6570\u4fdd\u969c\u817a\u764c\u6838\u5fc3\u6076\u6027\u7279\u5f81\u3001\u4e0a\u76ae\u5f62\u6001\u53ca\u589e\u6b96\u7a33\u5b9a\u6027\uff0c\u4f20\u4ee3\u8d85\u8fc7 15 \u4ee3\u9700\u91cd\u65b0\u9a8c\u8bc1 STR \u5206\u578b\u53ca\u5f62\u6001\u7eaf\u5ea6\uff09<\/li>\n<li><strong>\u4f7f\u7528\u6743\u9650<\/strong>\uff1aA \u7c7b\uff08\u9700\u9075\u5faa A \u7c7b\u7ec6\u80de\u4f7f\u7528\u89c4\u8303\uff0c\u9002\u914d\u79d1\u7814\u673a\u6784\u5408\u89c4\u6027\u5b9e\u9a8c\u9700\u6c42\uff0c\u5982\u5b9e\u9a8c\u65b9\u6848\u9700\u7b26\u5408\u6743\u9650\u5bf9\u5e94\u7684\u4f26\u7406\u53ca\u64cd\u4f5c\u6807\u51c6\uff09<\/li>\n<li><strong>\u7ec6\u80de\u89c4\u683c<\/strong>\uff1a1\u00d710\u2076cells\/T25 \u8d34\u58c1\u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u590d\u82cf\u540e\u5b58\u6d3b\u7387\u226580%\uff0c24 \u5c0f\u65f6\u8d34\u58c1\u7387\u226590%\uff0c\u9700\u955c\u68c0\u786e\u8ba4\u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001\u5360\u6bd4\uff08\u226585%\uff09\uff0c\u6392\u9664\u68ad\u5f62\u6742\u7ec6\u80de\u6c61\u67d3\u3002<\/li>\n<li><strong>\u652f\u539f\u4f53\u68c0\u6d4b<\/strong>\uff1a\u65e0\uff08\u7ecf PCR \u6cd5\u6216\u57f9\u517b\u6cd5\u68c0\u6d4b\uff0c\u786e\u8ba4\u65e0\u652f\u539f\u4f53\u6c61\u67d3\uff0c\u907f\u514d\u5f71\u54cd\u817a\u764c\u7ec6\u80de\u589e\u6b96\u53ca\u6297\u764c\u836f\u7269\u7b5b\u9009\u5b9e\u9a8c\u7ed3\u679c\u51c6\u786e\u6027\uff09<\/li>\n<li><strong>\u4fdd\u85cf\u673a\u6784<\/strong>\uff1a\u4e2d\u56fd\u5178\u578b\u57f9\u517b\u7269\u4fdd\u85cf\u4e2d\u5fc3\u7ec6\u80de\u5e93\uff08\u56fd\u5185\u6743\u5a01\u673a\u6784\u4fdd\u85cf\uff0c\u7ec6\u80de\u817a\u764c\u5c5e\u6027\u3001STR \u9274\u5b9a\u7ed3\u679c\u53ca\u4f7f\u7528\u6743\u9650\u7ecf\u9a8c\u8bc1\uff0c\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u9002\u914d\u56fd\u5185\u817a\u764c\u7814\u7a76\u5b9e\u9a8c\u5408\u89c4\u6027\u8981\u6c42\uff09<\/li>\n<li><strong>\u57f9\u517b\u57fa<\/strong>\uff1a90% DMEM \u57f9\u517b\u57fa + 10% FBS\uff08<strong>\u65e0\u989d\u5916\u6dfb\u52a0\u53cc\u6297\uff0c\u82e5\u9700\u9884\u9632\u6c61\u67d3\uff0c\u53ef\u6309\u9700\u6dfb\u52a0 1% \u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff08100U\/mL \u9752\u9709\u7d20 + 100\u03bcg\/mL \u94fe\u9709\u7d20\uff09<\/strong>\u00a0\uff09\uff1b\u4e0d\u53ef\u66ff\u6362\u4e3a F-12K \u6216 RPMI-1640\uff08DMEM \u9002\u914d\u901a\u7528\u817a\u764c\u7ec6\u80de\u4ee3\u8c22\u9700\u6c42\uff0c\u66ff\u6362\u540e\u6613\u5bfc\u81f4\u7ec6\u80de\u6d3b\u529b\u4e0b\u964d\u3001\u5f62\u6001\u5f02\u5e38\uff09\uff1b\u8840\u6e05\u9009\u4f4e\u5185\u6bd2\u7d20\u226410EU\/mL\uff0c\u5f00\u5c01\u540e 2 \u5468\u5185\u7528\u5b8c\uff0c\u4fdd\u969c\u7ec6\u80de\u5f62\u6001\u53ca\u589e\u6b96\u7a33\u5b9a\u3002<\/li>\n<li><strong>\u57f9\u517b\u6761\u4ef6<\/strong>\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff1b\u6bcf 48 \u5c0f\u65f6\u6362\u6db2 1 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